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Search for diarrheagenic Escherichia coli in raw kibbe samples reveals the presence of Shiga toxin-producing strains

dc.contributor.authorPeresi, Jacqueline Tanury Macruz
dc.contributor.authorde Almeida, Ivete Aparecida Zago Castanheira
dc.contributor.authorVaz, Tânia Mara Ibelli
dc.contributor.authorHernandes, Rodrigo Tavanelli [UNESP]
dc.contributor.authorde Carvalho Teixeira, Inara Siqueira
dc.contributor.authorde Lima e Silva, Sonia Izaura
dc.contributor.authorGraciano, Rejane Alexandre Silva
dc.contributor.authorPinheiro, Sandra Regina
dc.contributor.authordos Santos, Luis Fernando
dc.contributor.institutionCentro de Laboratório Regional de São José do Rio Preto
dc.contributor.institutionLaboratório Central - Centro de Bacteriologia
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:26:26Z
dc.date.available2018-12-11T17:26:26Z
dc.date.issued2016-05-01
dc.description.abstractThe aim of this study was to investigate the occurrence of diarrheagenic Escherichia coli (DEC) strains in raw kibbe samples. For this purpose, 70 samples of raw kibbe were collected from retail establishments and analyzed. Isolated bacterial strains that presented a biochemical profile of E. coli were screened by multiplex PCR for the genetic markers defining the main DEC pathotypes. Two strains belonging to O125:H19 and O149:H8 serotypes were positive for stx 1c genetic sequence and expressed the gene, being thus classified as Shiga-toxigenic E. coli (STEC). These strains were further characterized in respect to other virulence traits and susceptibility to antimicrobial agents. One of them presented the genes astA and lpfO113 and the other harbored only lpfO113. One of the strains was susceptible to all the antimicrobials tested but the other presented an intermediate profile of susceptibility to ampicillin. To the best of our knowledge this is probably the first description of O125:H19 and O149:H8 STEC serotypes in refrigerated raw kibbe and foods in general.en
dc.description.affiliationInstituto Adolfo Lutz Centro de Laboratório Regional de São José do Rio Preto, Rua Alberto Sufredine Bertone, 2325, Maceno
dc.description.affiliationInstituto Adolfo Lutz Laboratório Central - Centro de Bacteriologia, Av. Dr. Arnaldo, 355
dc.description.affiliationInstituto de Biociências Universidade Estadual Paulista (UNESP), Distrito de Rubião Junior, s/no
dc.description.affiliationUnespInstituto de Biociências Universidade Estadual Paulista (UNESP), Distrito de Rubião Junior, s/no
dc.format.extent165-170
dc.identifierhttp://dx.doi.org/10.1016/j.foodcont.2015.11.018
dc.identifier.citationFood Control, v. 63, p. 165-170.
dc.identifier.doi10.1016/j.foodcont.2015.11.018
dc.identifier.file2-s2.0-84949653383.pdf
dc.identifier.issn0956-7135
dc.identifier.lattes8376974115598584
dc.identifier.orcid0000-0001-6695-6003
dc.identifier.scopus2-s2.0-84949653383
dc.identifier.urihttp://hdl.handle.net/11449/177639
dc.language.isoeng
dc.relation.ispartofFood Control
dc.relation.ispartofsjr1,502
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAntimicrobial susceptibility profile
dc.subjectKibbe
dc.subjectShiga toxin-producing Escherichia coli
dc.subjectSTEC
dc.subjectVirulence markers
dc.titleSearch for diarrheagenic Escherichia coli in raw kibbe samples reveals the presence of Shiga toxin-producing strainsen
dc.typeArtigo
dspace.entity.typePublication
unesp.author.lattes8376974115598584[4]
unesp.author.orcid0000-0002-6158-5247[9]
unesp.author.orcid0000-0001-6695-6003[4]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatupt
unesp.departmentMicrobiologia e Imunologia - IBBpt

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