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Microsatellite loci in the oceanic whitetip shark and cross-species amplification using pyrosequencing technology

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dc.contributor.authorMendes, N. J. [UNESP]
dc.contributor.authorCruz, V. P. [UNESP]
dc.contributor.authorMendonça, Fernando Fernandes [UNESP]
dc.contributor.authorPardo, B. G.
dc.contributor.authorCoelho, R.
dc.contributor.authorAshikaga, F. Y. [UNESP]
dc.contributor.authorCamargo, S. M. [UNESP]
dc.contributor.authorMartinez, P.
dc.contributor.authorOliveira, C. [UNESP]
dc.contributor.authorSantos, M. N.
dc.contributor.authorForesti, F. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidad de Santiago de Compostela
dc.contributor.institutionInstituto Português do Mar e da Atmosfera (IPMA)
dc.contributor.institutionCentro de Ciências do Mar (CCMAR)
dc.date.accessioned2015-10-21T13:11:20Z
dc.date.available2015-10-21T13:11:20Z
dc.date.issued2015-06-01
dc.description.abstractAmong the pelagic shark species most impacted by fisheries, the oceanic whitetip shark (Carcharhinus longimanus) has shown signs of population declines. In this study, we developed 12 microsatellite markers using next generation sequencing (454 pyrosequencing) in C. longimanus for further use in population screening. The genetic diversity of these loci was assessed in 28 individuals and a total of 35 alleles were identified, ranging from four to eight alleles per locus (5.9 alleles on average). Cross-species amplification of these markers was successful in five loci for C. acronotus, C. perezi and Galeocerdo cuvier. The developed microsatellite markers will be useful in analyzing the population genetics structure of the oceanic whitetip shark and other Carcharhinidae species.en
dc.description.affiliationLaboratório Genética Pesqueira e Conservação, Instituto do Mar, Universidade Federal de São Paulo - UNIFESP, Av. Almirante Saldanha da Gama, 89. Ponta da Praia, Santos, SP, CEP 11030-400, Brazil
dc.description.affiliationDepartamento de Genética, Facultad de Veterinaria, Universidad de Santiago de Compostela, 27002, Lugo, Spain
dc.description.affiliationInstituto Português do Mar e da Atmosfera, IPMA, I.P., Olhão, Portugal
dc.description.affiliationCentro de Ciências do Mar, CCMAR, Universidade Algarve, Faro, Portugal
dc.description.affiliationUnespUniversidade Estadual Paulista, Laboratório de Biologia e Genética de Peixes, Instituto de Biociências de Botucatu, UNESP, Botucatu, SP, 18618-970, Brazil
dc.description.sponsorshipFCT
dc.description.sponsorshipPortuguese Foundation for Science and Technology
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdPortuguese Foundation for Science and Technology: SFRH/BPD/93936/2013
dc.description.sponsorshipIdFAPESP: 2013/14555-4
dc.description.sponsorshipIdFAPESP: 2011/23787-0
dc.description.sponsorshipIdFAPESP: 2010/51903-2
dc.format.extent585-589
dc.identifierhttp://link.springer.com/article/10.1007%2Fs12686-015-0435-5
dc.identifier.citationConservation Genetics Resources. Dordrecht: Springer, v. 7, n. 2, p. 585-589, 2015.
dc.identifier.doi10.1007/s12686-015-0435-5
dc.identifier.issn1877-7252
dc.identifier.lattes0804793944846367
dc.identifier.urihttp://hdl.handle.net/11449/128596
dc.identifier.wosWOS:000354213300069
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofConservation Genetics Resources
dc.relation.ispartofjcr0.742
dc.relation.ispartofsjr0,430
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectConservationen
dc.subjectPopulation geneticsen
dc.subjectNext generation sequencingen
dc.subjectCarcharhinusen
dc.titleMicrosatellite loci in the oceanic whitetip shark and cross-species amplification using pyrosequencing technologyen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
dspace.entity.typePublication
unesp.author.lattes0804793944846367
unesp.author.orcid0000-0003-3813-5157[5]
unesp.author.orcid0000-0002-4806-9897[3]
unesp.author.orcid0000-0003-3548-6306[4]
unesp.author.orcid0000-0001-8438-9305[8]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatupt
unesp.departmentGenética - IBBpt

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Botucatu - IBB - Instituto de Biociências