Publicação: Localization and hormonal regulation of endometrial matrix metalloproteinase-26 in the rhesus macaque
dc.contributor.author | Almeida-Francia, C. C. D. [UNESP] | |
dc.contributor.author | Keator, C. S. | |
dc.contributor.author | Mah, K. | |
dc.contributor.author | Holden, L. | |
dc.contributor.author | Hergert, C. | |
dc.contributor.author | Slayden, O. D. | |
dc.contributor.institution | Oregon Hlth & Sci Univ | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Ross Univ | |
dc.date.accessioned | 2014-05-20T15:33:24Z | |
dc.date.available | 2014-05-20T15:33:24Z | |
dc.date.issued | 2012-06-01 | |
dc.description.abstract | The current understanding of hormonal regulation of matrix metalloproteinase-26 (MMP-26) in the primate endometrium is incomplete. The goal of this work was to clarify estrogen and progesterone regulation of MMP-26 in the endometrium of ovariectomized, hormone-treated rhesus macaques.Ovariectomized rhesus macaques (n 66) were treated with estradiol (E-2), E-2 plus progesterone, E-2 followed by progesterone alone or no hormone. Endometrium was collected from the hormone-treated animals during the early, mid- and late proliferative and secretory phases of the artificial menstrual cycle. MMP-26 expression was quantified by real-time PCR, and MMP-26 transcript and protein were localized by in situ hybridization and immunohistochemistry and correlated with estrogen receptor 1 and progesterone receptor (PGR).MMP-26 was localized to glandular epithelium and was undetectable in the endometrial stroma and vasculature. MMP-26 transcript levels were minimal in the hormone-deprived macaques and treatment with E-2 alone did not affect MMP-26 levels. Treatment with progesterone both in the presence and absence of E-2 stimulated MMP-26 expression in the early and mid-secretory phases (P 0.001). MMP-26 expression preceded decidualization of endometrial stroma. MMP-26 levels then declined to baseline in the late secretory phase (P 0.01) despite continued E-2 plus progesterone treatment. Loss of detectable MMP-26 expression in the late secretory phase was correlated with late secretory phase loss of glandular epithelial PGR.Endometrial MMP-26 expression is dependent on the presence of progesterone in the early secretory phase and then gradually becomes refractory to progesterone stimulation in the late secretory phase. In the macaque, MMP-26 is a marker of the pre-decidual, secretory endometrium. During the second half of the late secretory phase, and during decidualization, MMP-26 loses its response to progesterone concurrent with the loss of epithelial PGR. The decline in MMP-26 levels between the mid- and late secretory phases may play a role in the receptive window for embryo implantation. | en |
dc.description.affiliation | Oregon Hlth & Sci Univ, Oregon Natl Primate Res Ctr, Div Reprod & Dev Sci, Beaverton, OR 97006 USA | |
dc.description.affiliation | Univ Estadual Paulista UNESP, Inst Biosci, Dept Anat, BR-18618970 São Paulo, Brazil | |
dc.description.affiliation | Ross Univ, Sch Med, Dept Physiol, Picard, Commonwealth Of, W Ind Assoc St | |
dc.description.affiliationUnesp | Univ Estadual Paulista UNESP, Inst Biosci, Dept Anat, BR-18618970 São Paulo, Brazil | |
dc.description.sponsorship | NIH | |
dc.description.sponsorship | NIH Fogarty International Center | |
dc.description.sponsorshipId | NIH: HD18185 | |
dc.description.sponsorshipId | NIH: RR000163 | |
dc.description.sponsorshipId | NIH: TW/HD-00668 | |
dc.format.extent | 1723-1734 | |
dc.identifier | http://dx.doi.org/10.1093/humrep/des086 | |
dc.identifier.citation | Human Reproduction. Oxford: Oxford Univ Press, v. 27, n. 6, p. 1723-1734, 2012. | |
dc.identifier.doi | 10.1093/humrep/des086 | |
dc.identifier.issn | 0268-1161 | |
dc.identifier.uri | http://hdl.handle.net/11449/42035 | |
dc.identifier.wos | WOS:000304530900023 | |
dc.language.iso | eng | |
dc.publisher | Oxford University Press | |
dc.relation.ispartof | Human Reproduction | |
dc.relation.ispartofjcr | 4.990 | |
dc.relation.ispartofsjr | 2,643 | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | matrix metalloproteinase-26 | en |
dc.subject | estradiol | en |
dc.subject | progesterone | en |
dc.subject | rhesus macaque | en |
dc.subject | endometrium | en |
dc.title | Localization and hormonal regulation of endometrial matrix metalloproteinase-26 in the rhesus macaque | en |
dc.type | Artigo | |
dcterms.license | http://www.oxfordjournals.org/access_purchase/self-archiving_policyb1.html | |
dcterms.rightsHolder | Oxford Univ Press | |
dspace.entity.type | Publication | |
unesp.author.lattes | 4791367810581192[1] | |
unesp.author.orcid | 0000-0003-0832-6083[1] | |
unesp.campus | Universidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatu | pt |
unesp.department | Anatomia - IBB | pt |
Arquivos
Licença do Pacote
1 - 2 de 2
Carregando...
- Nome:
- license.txt
- Tamanho:
- 1.71 KB
- Formato:
- Item-specific license agreed upon to submission
- Descrição:
Carregando...
- Nome:
- license.txt
- Tamanho:
- 1.71 KB
- Formato:
- Item-specific license agreed upon to submission
- Descrição: