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Localization and hormonal regulation of endometrial matrix metalloproteinase-26 in the rhesus macaque

dc.contributor.authorAlmeida-Francia, C. C. D. [UNESP]
dc.contributor.authorKeator, C. S.
dc.contributor.authorMah, K.
dc.contributor.authorHolden, L.
dc.contributor.authorHergert, C.
dc.contributor.authorSlayden, O. D.
dc.contributor.institutionOregon Hlth & Sci Univ
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionRoss Univ
dc.date.accessioned2014-05-20T15:33:24Z
dc.date.available2014-05-20T15:33:24Z
dc.date.issued2012-06-01
dc.description.abstractThe current understanding of hormonal regulation of matrix metalloproteinase-26 (MMP-26) in the primate endometrium is incomplete. The goal of this work was to clarify estrogen and progesterone regulation of MMP-26 in the endometrium of ovariectomized, hormone-treated rhesus macaques.Ovariectomized rhesus macaques (n 66) were treated with estradiol (E-2), E-2 plus progesterone, E-2 followed by progesterone alone or no hormone. Endometrium was collected from the hormone-treated animals during the early, mid- and late proliferative and secretory phases of the artificial menstrual cycle. MMP-26 expression was quantified by real-time PCR, and MMP-26 transcript and protein were localized by in situ hybridization and immunohistochemistry and correlated with estrogen receptor 1 and progesterone receptor (PGR).MMP-26 was localized to glandular epithelium and was undetectable in the endometrial stroma and vasculature. MMP-26 transcript levels were minimal in the hormone-deprived macaques and treatment with E-2 alone did not affect MMP-26 levels. Treatment with progesterone both in the presence and absence of E-2 stimulated MMP-26 expression in the early and mid-secretory phases (P 0.001). MMP-26 expression preceded decidualization of endometrial stroma. MMP-26 levels then declined to baseline in the late secretory phase (P 0.01) despite continued E-2 plus progesterone treatment. Loss of detectable MMP-26 expression in the late secretory phase was correlated with late secretory phase loss of glandular epithelial PGR.Endometrial MMP-26 expression is dependent on the presence of progesterone in the early secretory phase and then gradually becomes refractory to progesterone stimulation in the late secretory phase. In the macaque, MMP-26 is a marker of the pre-decidual, secretory endometrium. During the second half of the late secretory phase, and during decidualization, MMP-26 loses its response to progesterone concurrent with the loss of epithelial PGR. The decline in MMP-26 levels between the mid- and late secretory phases may play a role in the receptive window for embryo implantation.en
dc.description.affiliationOregon Hlth & Sci Univ, Oregon Natl Primate Res Ctr, Div Reprod & Dev Sci, Beaverton, OR 97006 USA
dc.description.affiliationUniv Estadual Paulista UNESP, Inst Biosci, Dept Anat, BR-18618970 São Paulo, Brazil
dc.description.affiliationRoss Univ, Sch Med, Dept Physiol, Picard, Commonwealth Of, W Ind Assoc St
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Inst Biosci, Dept Anat, BR-18618970 São Paulo, Brazil
dc.description.sponsorshipNIH
dc.description.sponsorshipNIH Fogarty International Center
dc.description.sponsorshipIdNIH: HD18185
dc.description.sponsorshipIdNIH: RR000163
dc.description.sponsorshipIdNIH: TW/HD-00668
dc.format.extent1723-1734
dc.identifierhttp://dx.doi.org/10.1093/humrep/des086
dc.identifier.citationHuman Reproduction. Oxford: Oxford Univ Press, v. 27, n. 6, p. 1723-1734, 2012.
dc.identifier.doi10.1093/humrep/des086
dc.identifier.issn0268-1161
dc.identifier.urihttp://hdl.handle.net/11449/42035
dc.identifier.wosWOS:000304530900023
dc.language.isoeng
dc.publisherOxford University Press
dc.relation.ispartofHuman Reproduction
dc.relation.ispartofjcr4.990
dc.relation.ispartofsjr2,643
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectmatrix metalloproteinase-26en
dc.subjectestradiolen
dc.subjectprogesteroneen
dc.subjectrhesus macaqueen
dc.subjectendometriumen
dc.titleLocalization and hormonal regulation of endometrial matrix metalloproteinase-26 in the rhesus macaqueen
dc.typeArtigo
dcterms.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb1.html
dcterms.rightsHolderOxford Univ Press
dspace.entity.typePublication
unesp.author.lattes4791367810581192[1]
unesp.author.orcid0000-0003-0832-6083[1]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatupt
unesp.departmentAnatomia - IBBpt

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