Transference of a crystal protein gene from bacillus thuringiensis and its expression in bradyrhizobium sp. cells
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Undergraduate course
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Abstract
The plasmid pHT409 that harbours the cryIA(a) gene for the production of a δ-endotoxin (crystal protein) from Bacillus thuringiensis was transferred into Bradyrhizobium sp. A conjugal transfer system aiming to introduce the plasmid into the Bradyrhizobium sp. host from colonies of an Escherichia coli donor strain (DH5α
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Keywords
Bacillus thuringiensis, Bradyrhizobium sp, Conjugation, cryIA (a), Crystal protein, Plasmid stability, crystal protein, gene transfer, genetic strain, plasmid
Language
English
Citation
World Journal of Microbiology and Biotechnology, v. 16, n. 4, p. 361-365, 2000.
