The peptide secreted at the water to land transition in a model amphibian has antioxidant effects
dc.contributor.author | Barbosa, Eder Alves | |
dc.contributor.author | Plácido, Alexandra | |
dc.contributor.author | Moreira, Daniel C. | |
dc.contributor.author | Albuquerque, Lucas | |
dc.contributor.author | Dematei, Anderson | |
dc.contributor.author | Silva-Carvalho, Amandda É. | |
dc.contributor.author | Cabral, Wanessa F. | |
dc.contributor.author | Báo, Sonia N. | |
dc.contributor.author | Saldanha-Araújo, Felipe | |
dc.contributor.author | Kuckelhaus, Selma A. S. | |
dc.contributor.author | Borges, Tatiana K. | |
dc.contributor.author | Portugal, Camila C. | |
dc.contributor.author | Socodato, Renato | |
dc.contributor.author | Teixeira, Cátia | |
dc.contributor.author | Lima, Filipe Camargo D. A. | |
dc.contributor.author | Batagin-Neto, Augusto [UNESP] | |
dc.contributor.author | Sebben, Antônio | |
dc.contributor.author | Eaton, Peter | |
dc.contributor.author | Gomes, Paula | |
dc.contributor.author | Brand, Guilherme D. | |
dc.contributor.author | Relvas, Joao B. | |
dc.contributor.author | Kato, Massuo J. | |
dc.contributor.author | Leite, Jose Roberto S. A. | |
dc.contributor.institution | Universidade de Brasília (UnB) | |
dc.contributor.institution | Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) | |
dc.contributor.institution | UPTEC | |
dc.contributor.institution | Universidade Do Porto | |
dc.contributor.institution | Ciência e Tecnologia de São Paulo | |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | |
dc.contributor.institution | University of Lincoln | |
dc.contributor.institution | Universidade de São Paulo (USP) | |
dc.date.accessioned | 2022-04-29T08:36:48Z | |
dc.date.available | 2022-04-29T08:36:48Z | |
dc.date.issued | 2021-01-01 | |
dc.description.abstract | In addition to the morphophysiological changes experienced by amphibians during metamorphosis, they must also deal with a different set of environmental constraints when they shift from the water to the land. We found that Pithecopus azureus secretes a single peptide ([M + H]+ = 658.38 Da) at the developmental stage that precedes the onset of terrestrial behaviour. De novo peptide and cDNA sequencing revealed that the peptide, named PaT-2, is expressed in tandem and is a member of the tryptophyllins family. In silico studies allowed us to identify the position of reactive sites and infer possible antioxidant mechanisms of the compounds. Cell-based assays confirmed the predicted antioxidant activity in mammalian microglia and neuroblast cells. The potential neuroprotective effect of PaT-2 was further corroborated in FRET-based live cell imaging assays, where the peptide prevented lipopolysaccharide-induced ROS production and glutamate release in human microglia. In summary, PaT-2 is the first peptide expressed during the ontogeny of P. azureus, right before the metamorphosing froglet leaves the aquatic environment to occupy terrestrial habitats. The antioxidant activity of PaT-2, predicted by in silico analyses and confirmed by cell-based assays, might be relevant for the protection of the skin of P. azureus adults against increased O 2 levels and UV exposure on land compared with aquatic environments. | en |
dc.description.affiliation | Laboratório de Síntese e Análise de Biomoléculas Instituto de Química Universidade de Brasília | |
dc.description.affiliation | Núcleo de Pesquisa em Morfologia e Imunologia Aplicada (NuPMIA) Faculdade de Medicina Universidade de Brasília | |
dc.description.affiliation | Laboratório de Imunologia Celular NuPMIA Faculdade de Medicina Universidade de Brasília | |
dc.description.affiliation | Programa de Pós-graduação em Medicina Tropical Núcleo de Medicina Tropical Faculdade de Medicina Universidade de Brasília | |
dc.description.affiliation | Laboratório de Hematologia e Celulas-tronco Faculdade de Ciências da Saúde Universidade de Brasília | |
dc.description.affiliation | Laboratório de Microscopia e Microanálise Instituto de Ciências Biológicas Universidade de Brasília | |
dc.description.affiliation | Instituto de Ciências Biológicas Universidade de Brasília | |
dc.description.affiliation | Laboratório de Espectrometria de Massa Embrapa Recursos Genéticos e Biotecnologia | |
dc.description.affiliation | Bioprospectum Lda UPTEC | |
dc.description.affiliation | LAQV/REQUIMTE Departamento de Química e Bioquímica Faculdade de Ciências Universidade Do Porto | |
dc.description.affiliation | Glial Cell Biology Lab Instituto de Investigacao e Inovacao em Saude and Instituto de Biologia Molecular e Celular Universidade Do Porto | |
dc.description.affiliation | Instituto Federal de Educação Ciência e Tecnologia de São Paulo | |
dc.description.affiliation | São Paulo State University (Unesp) Campus of Itapeva | |
dc.description.affiliation | The Bridge School of Chemistry Joseph Banks Laboratories University of Lincoln | |
dc.description.affiliation | Departamento de Química Fundamental Instituto de Química Universidade de São Paulo, SP | |
dc.description.affiliationUnesp | São Paulo State University (Unesp) Campus of Itapeva | |
dc.identifier | http://dx.doi.org/10.1098/rspb.2021.1531 | |
dc.identifier.citation | Proceedings of the Royal Society B: Biological Sciences, v. 288, n. 1962, 2021. | |
dc.identifier.doi | 10.1098/rspb.2021.1531 | |
dc.identifier.issn | 1471-2954 | |
dc.identifier.issn | 0962-8452 | |
dc.identifier.scopus | 2-s2.0-85119997100 | |
dc.identifier.uri | http://hdl.handle.net/11449/229957 | |
dc.language.iso | eng | |
dc.relation.ispartof | Proceedings of the Royal Society B: Biological Sciences | |
dc.source | Scopus | |
dc.subject | Amphibia | |
dc.subject | antioxidant peptide | |
dc.subject | MALDI mass spectrometry imaging | |
dc.subject | oxidative stress | |
dc.subject | Pithecopus azureus | |
dc.subject | tryptophyllin | |
dc.title | The peptide secreted at the water to land transition in a model amphibian has antioxidant effects | en |
dc.type | Artigo | |
dspace.entity.type | Publication | |
unesp.author.orcid | 0000-0002-7613-4414 0000-0002-7613-4414[1] | |
unesp.author.orcid | 0000-0003-2706-7777 0000-0003-2706-7777[2] | |
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unesp.author.orcid | 0000-0002-3315-2129[22] | |
unesp.author.orcid | 0000-0002-1096-3236 0000-0002-1096-3236[23] | |
unesp.campus | Universidade Estadual Paulista (UNESP), Instituto de Ciências e Engenharia, Itapeva | pt |
unesp.department | Engenharia Industrial Madeireira - ICE | pt |