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The peptide secreted at the water to land transition in a model amphibian has antioxidant effects

dc.contributor.authorBarbosa, Eder Alves
dc.contributor.authorPlácido, Alexandra
dc.contributor.authorMoreira, Daniel C.
dc.contributor.authorAlbuquerque, Lucas
dc.contributor.authorDematei, Anderson
dc.contributor.authorSilva-Carvalho, Amandda É.
dc.contributor.authorCabral, Wanessa F.
dc.contributor.authorBáo, Sonia N.
dc.contributor.authorSaldanha-Araújo, Felipe
dc.contributor.authorKuckelhaus, Selma A. S.
dc.contributor.authorBorges, Tatiana K.
dc.contributor.authorPortugal, Camila C.
dc.contributor.authorSocodato, Renato
dc.contributor.authorTeixeira, Cátia
dc.contributor.authorLima, Filipe Camargo D. A.
dc.contributor.authorBatagin-Neto, Augusto [UNESP]
dc.contributor.authorSebben, Antônio
dc.contributor.authorEaton, Peter
dc.contributor.authorGomes, Paula
dc.contributor.authorBrand, Guilherme D.
dc.contributor.authorRelvas, Joao B.
dc.contributor.authorKato, Massuo J.
dc.contributor.authorLeite, Jose Roberto S. A.
dc.contributor.institutionUniversidade de Brasília (UnB)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionUPTEC
dc.contributor.institutionUniversidade Do Porto
dc.contributor.institutionCiência e Tecnologia de São Paulo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of Lincoln
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2022-04-29T08:36:48Z
dc.date.available2022-04-29T08:36:48Z
dc.date.issued2021-01-01
dc.description.abstractIn addition to the morphophysiological changes experienced by amphibians during metamorphosis, they must also deal with a different set of environmental constraints when they shift from the water to the land. We found that Pithecopus azureus secretes a single peptide ([M + H]+ = 658.38 Da) at the developmental stage that precedes the onset of terrestrial behaviour. De novo peptide and cDNA sequencing revealed that the peptide, named PaT-2, is expressed in tandem and is a member of the tryptophyllins family. In silico studies allowed us to identify the position of reactive sites and infer possible antioxidant mechanisms of the compounds. Cell-based assays confirmed the predicted antioxidant activity in mammalian microglia and neuroblast cells. The potential neuroprotective effect of PaT-2 was further corroborated in FRET-based live cell imaging assays, where the peptide prevented lipopolysaccharide-induced ROS production and glutamate release in human microglia. In summary, PaT-2 is the first peptide expressed during the ontogeny of P. azureus, right before the metamorphosing froglet leaves the aquatic environment to occupy terrestrial habitats. The antioxidant activity of PaT-2, predicted by in silico analyses and confirmed by cell-based assays, might be relevant for the protection of the skin of P. azureus adults against increased O 2 levels and UV exposure on land compared with aquatic environments.en
dc.description.affiliationLaboratório de Síntese e Análise de Biomoléculas Instituto de Química Universidade de Brasília
dc.description.affiliationNúcleo de Pesquisa em Morfologia e Imunologia Aplicada (NuPMIA) Faculdade de Medicina Universidade de Brasília
dc.description.affiliationLaboratório de Imunologia Celular NuPMIA Faculdade de Medicina Universidade de Brasília
dc.description.affiliationPrograma de Pós-graduação em Medicina Tropical Núcleo de Medicina Tropical Faculdade de Medicina Universidade de Brasília
dc.description.affiliationLaboratório de Hematologia e Celulas-tronco Faculdade de Ciências da Saúde Universidade de Brasília
dc.description.affiliationLaboratório de Microscopia e Microanálise Instituto de Ciências Biológicas Universidade de Brasília
dc.description.affiliationInstituto de Ciências Biológicas Universidade de Brasília
dc.description.affiliationLaboratório de Espectrometria de Massa Embrapa Recursos Genéticos e Biotecnologia
dc.description.affiliationBioprospectum Lda UPTEC
dc.description.affiliationLAQV/REQUIMTE Departamento de Química e Bioquímica Faculdade de Ciências Universidade Do Porto
dc.description.affiliationGlial Cell Biology Lab Instituto de Investigacao e Inovacao em Saude and Instituto de Biologia Molecular e Celular Universidade Do Porto
dc.description.affiliationInstituto Federal de Educação Ciência e Tecnologia de São Paulo
dc.description.affiliationSão Paulo State University (Unesp) Campus of Itapeva
dc.description.affiliationThe Bridge School of Chemistry Joseph Banks Laboratories University of Lincoln
dc.description.affiliationDepartamento de Química Fundamental Instituto de Química Universidade de São Paulo, SP
dc.description.affiliationUnespSão Paulo State University (Unesp) Campus of Itapeva
dc.identifierhttp://dx.doi.org/10.1098/rspb.2021.1531
dc.identifier.citationProceedings of the Royal Society B: Biological Sciences, v. 288, n. 1962, 2021.
dc.identifier.doi10.1098/rspb.2021.1531
dc.identifier.issn1471-2954
dc.identifier.issn0962-8452
dc.identifier.scopus2-s2.0-85119997100
dc.identifier.urihttp://hdl.handle.net/11449/229957
dc.language.isoeng
dc.relation.ispartofProceedings of the Royal Society B: Biological Sciences
dc.sourceScopus
dc.subjectAmphibia
dc.subjectantioxidant peptide
dc.subjectMALDI mass spectrometry imaging
dc.subjectoxidative stress
dc.subjectPithecopus azureus
dc.subjecttryptophyllin
dc.titleThe peptide secreted at the water to land transition in a model amphibian has antioxidant effectsen
dc.typeArtigo
dspace.entity.typePublication
unesp.author.orcid0000-0002-7613-4414 0000-0002-7613-4414[1]
unesp.author.orcid0000-0003-2706-7777 0000-0003-2706-7777[2]
unesp.author.orcid0000-0003-1961-7281[3]
unesp.author.orcid0000-0001-7164-2850[4]
unesp.author.orcid0000-0003-4270-3567 0000-0003-4270-3567[5]
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unesp.author.orcid0000-0002-6813-6276[11]
unesp.author.orcid0000-0002-1093-6545[12]
unesp.author.orcid0000-0002-6882-5020[13]
unesp.author.orcid0000-0001-9506-3781[14]
unesp.author.orcid0000-0001-7062-5450[15]
unesp.author.orcid0000-0003-4609-9002[16]
unesp.author.orcid0000-0003-1821-1992 0000-0003-1821-1992[18]
unesp.author.orcid0000-0002-6018-4724[19]
unesp.author.orcid0000-0002-1615-0009[20]
unesp.author.orcid0000-0001-7636-0924[21]
unesp.author.orcid0000-0002-3315-2129[22]
unesp.author.orcid0000-0002-1096-3236 0000-0002-1096-3236[23]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Ciências e Engenharia, Itapevapt
unesp.departmentEngenharia Industrial Madeireira - ICEpt

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