Postfire Alterations of the Resin Secretory System in Protium heptaphyllum (Aubl.) Marchand (Burseraceae)

Abstract

Fire is a natural disturbance in the Brazilian Cerrado that modulates the vegetation structure. Protium heptaphyllum, a woody species of the family Burseraceae, is common in this biome. The resin produced in secretory canals immersed in the phloem of the stem and leaves of this species plays important ecological and industrial roles. The aim of this study was to investigate the influence of fire on the development of resin canals in the leaves and stem of P. heptaphyllum and on the chemical profile of substances produced in the leaves. Young plants were subjected to controlled fire experiments. Leaf and stem portions were analyzed using light microscopy; the chemical compounds in the leaves were identified through gas chromatography–mass spectrometry. The percentage area occupied by secretory canals in the leaf midrib was higher in fire-treated plants than in control plants. Similarly, the density of secretory canals and their lumen area were higher in young stems (primary growth) of fire-treated plants. By contrast, although the canal density in the secondary phloem was lower in older stem portions (secondary growth) in fire-treated plants, their lumens were larger, resulting in similar data regarding the total lumen area of the secretory canals in fire-treated and control plants. The main chemical compounds identified in the leaves were vitamin E, sitosterol, α-amyrin, squalene, and β-amyrin. Three compounds showed significant increases in fire-treated plants, with vitamin E being the only one reduced by fire. Our findings reveal the plasticity of the secretory system and of the biochemical properties of the leaves of P. heptaphyllum in response to fire. These results are important when considering the current increase in fires caused by climate change and human activity in different ecosystems around the world.