The zymogen-enteropeptidase system: A practical approach to study the regulation of enzyme activity by proteolytic cleavage
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Graduate program
Undergraduate course
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Publisher
Amer Soc Biochemistry Molecular Biology Inc
Type
Article
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Acesso aberto
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Abstract
The present research describes an efficient procedure to obtain high levels of trypsinogen and chymotrypsinogen by using a simple, rapid, and easily reproducible method. The extraction process and the time-course of activation of zymogens can be carried out in a single laboratory period, without sophisticated equipment. The main objective was to prepare a laboratory class that would stimulate student interest in enzyme regulation, exploring the fact that the catalytic activity of some enzymes is regulated by different mechanisms. The regulation of proteolytic enzymes requires the synthesis of an inactive zymogen and its being irreversibly switched on by specific proteolytic cleavage.
Description
Keywords
zymogen, trypsin, chymotrypsin, enteropeptidase, enzyme regulation, proteolytic cleavage
Language
English
Citation
Biochemistry and Molecular Biology Education. Bethesda: Amer Soc Biochemistry Molecular Biology Inc., v. 32, n. 1, p. 45-48, 2004.
