Publicação: Quality evaluation of decellularized blood vessels
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Data
2014-12-08
Autores
Orientador
Reis, Patricia Pintor dos 
Deffune, Elenice
Coorientador
Pós-graduação
Curso de graduação
Ciências Biológicas - IBB
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Universidade Estadual Paulista (Unesp)
Tipo
Trabalho de conclusão de curso
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Acesso aberto
Resumo
Resumo (inglês)
Currently, it has been used biological media derived from decellularized tissue and organs in studies, since they have biological advantages. However, it is necessary to make a thorough preparation to ensure the decellularization. Aims: To evaluate the possible existence of DNA in decellularized vessels. Methods: Samples are to infrarenal vena veins of rabbits treated with 1) Sodium deoxycholate (DS) 2% and 2) Sodium dodecyl sulfate (SDS) 1%, with in natura veins as control. After decellularization, the DNA was extracted and quantified by NanoDrop. Thereafter was analyzed on agarose gel. Results: Both evaluated decellularization techniques have DNA remnant. Conclusion: The method of decellularization with SDS shown more efficient that using DS. However, additional studies of optimization and quality control are necessary to ensure the absence of DNA in scaffolds, minimizing problems such as tissue rejection
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Português
Como citar
BOVOLATO, Ana Lívia de Carvalho. Quality evaluation of decellularized blood vessels. 2014. 1 CD-ROM. Trabalho de conclusão de curso (bacharelado - Ciências Biológicas) - Universidade Estadual Paulista Júlio de Mesquita Filho, Instituto de Biociências de Botucatu, 2014.
