Publicação: Quantitative distribution of gibberellins and indole-acetic acid in pea (Pisum sativum L.) seedlings
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Fundacion Romulo Raggio
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The objective was to determine the distribution in pea seedling of GA (by thin layer chromatography) and IAA (by HPLC). Three samples of 30 7/day-old seedlings were sectioned into: apical meristem + plumular hook; stem elongation zone; stem non-elongation zone; cotyledons; root non-elongation zone; root tip. The material was frozen in liquid N and stored at -80 degrees C. The stem elongation zone, the apical meristem+plumular hook and the root tip had low IAA, whereas the stem non-elongation zone and root non-elongation zone had high IAA content. The stem elongation zone and apical meristem plumular hook had high GA, while root non-elongation zone had the lowest GA content.
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HPLC, TLC, gibberellic acid, IAA
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Inglês
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Phyton-international Journal of Experimental Botany. Vicente Lopez (ba): Fundacion Romulo Raggio, v. 63, n. 1-2, p. 75-80, 1998.
