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A trypsin inhibitor from Moringa oleifera flower extract is cytotoxic to Trypanosoma cruzi with high selectivity over mammalian cells

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dc.contributor.authorPontual, Emmanuel V.
dc.contributor.authorPires-Neto, Divar F.
dc.contributor.authorFraige, Karina [UNESP]
dc.contributor.authorHigino, Taciana M. M.
dc.contributor.authorCarvalho, Belany E. A.
dc.contributor.authorAlves, Neyla M. P.
dc.contributor.authorLima, Thamarah A.
dc.contributor.authorZingali, Russolina B.
dc.contributor.authorCoelho, Luana C. B. B.
dc.contributor.authorBolzani, Vanderlan S. [UNESP]
dc.contributor.authorFigueiredo, Regina C. B. Q.
dc.contributor.authorNapoleao, Thiago H.
dc.contributor.authorPaiva, Patricia M. G.
dc.contributor.institutionUniv Fed Rural Pernambuco
dc.contributor.institutionFundacao Oswaldo Cruz
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionUniv Fed Rio Janeiro
dc.date.accessioned2019-10-04T12:35:55Z
dc.date.available2019-10-04T12:35:55Z
dc.date.issued2018-12-17
dc.description.abstractIn this study, Moringa oleifera flower extract and a trypsin inhibitor (MoFTI) isolated from it were evaluated for anti-protozoal activity against Trypanosoma cruzi and cytotoxicity to mammalian cells. The presence of flavonoids was remarkable in the HPLC fingerprints of the extract at 254 and 360 nm. Amino acid sequences of peptides derived from in-gel digestion of MoFTI were determined. Both the extract and MoFTI caused lysis of T. cruzi trypomastigotes with LC50/24 h of 54.18 +/- 6.62 and 41.20 +/- 4.28 mu g/mL, respectively. High selectivity indices (7.9 to >12) for T. cruzi cells over murine peritoneal macrophages and Vero cells were found for the extract and MoFTI. The results show that MoFTI is a trypanocidal principle of the flower extract. [GRAPHICS] .en
dc.description.affiliationUniv Fed Rural Pernambuco, Dept Morfol & Fisiol Anim, Recife, PE, Brazil
dc.description.affiliationFundacao Oswaldo Cruz, Ctr Pesquisas Aggeu Magalhaes, Dept Microbiol, Recife, PE, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Quim, Dept Quim Organ, Araraquara, Brazil
dc.description.affiliationUniv Fed Pernambuco, Ctr Ciencias Biol, Dept Bioquim, Recife, PE, Brazil
dc.description.affiliationUniv Fed Rio Janeiro, Inst Bioquim Med Leopoldo de Meis, Rio De Janeiro, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim, Dept Quim Organ, Araraquara, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE)
dc.description.sponsorshipIdCNPq: 472546/2012-0
dc.description.sponsorshipIdCNPq: 446902/2014-4
dc.description.sponsorshipIdFundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE): APQ-0108-2.08/14
dc.format.extent2940-2944
dc.identifierhttp://dx.doi.org/10.1080/14786419.2017.1389932
dc.identifier.citationNatural Product Research. Abingdon: Taylor & Francis Ltd, v. 32, n. 24, p. 2940-2944, 2018.
dc.identifier.doi10.1080/14786419.2017.1389932
dc.identifier.issn1478-6419
dc.identifier.urihttp://hdl.handle.net/11449/185484
dc.identifier.wosWOS:000459884600012
dc.language.isoeng
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofNatural Product Research
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectMoringa oleifera
dc.subjectchagas disease
dc.subjectprotease inhibitor
dc.subjecttrypomastigote
dc.subjectcytotoxicity
dc.titleA trypsin inhibitor from Moringa oleifera flower extract is cytotoxic to Trypanosoma cruzi with high selectivity over mammalian cellsen
dc.typeArtigo
dcterms.licensehttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dcterms.rightsHolderTaylor & Francis Ltd
dspace.entity.typePublication
unesp.author.orcid0000-0002-8298-6568[3]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Química, Araraquarapt
unesp.departmentQuímica Orgânica - IQARpt

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