Utilização de meios alternativos e diferentes tipos de recipientes no cultivo de Ankistrodesmus gracilis (Reinsch) Korshikov (Chlorophyceae) em laboratório
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Universidade Estadual de Maringa
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Resumo
A laboratory culture of Ankistrodesmus gracilis algae was evaluated by studying the biology of the species and its chemical composition in a traditional medium (CHU 12) and in two alternative culture media, NPK (20-5-20) and macrophyte (Eichhornia crassipes) + NPK, in three different types of recipients (fiberglass, carboy and plastic bag). First peak in the growth curve of Ankistrodesmus gracilis occurred on the ninth day in macrophyte + NPK medium (74.16 x 10 5 cells mL -1) in a fiberglass recipient. However, highest density (p < 0.01) was reported in medium CHU 12 (122.87 x 10 5 cells mL -1) in a plastic bag on the twelfth day. Cell density was over 70 x 10 5 cells mL -1 starting on the twelfth day. Growth rate of A. gracilis was similar (p > 0.05) in culture media in the three recipients. Protein and fiber were similar (p > 0.05) in the treatments, but lipids were higher (p < 0.05) in NPK. Nitrate, ammonia, total phosphorus and orthophosphate contents were over 1 mg L -1 in NPK (p < 0.01). Results show that alternative media, such as NPK and macrophyte + NPK, are possible for large-scale culture of A. gracilis cultured in three types of recipients. Costs are low, occupying less space when cultured in plastic bags and in the laboratory.
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Algae , Biochemical composition , CHU 12 , Growth , Macrophyte medium , NPK , ammonia , glass fiber , lipid , nitrate , phosphate , phosphorus , plastic , algal cell culture , algal growth , carboy , cell density , chemical composition , Chlorophyceae , CHU12 , container , controlled study , culture medium , Eichhornia , growth curve , growth rate , laboratory , macrophyte , microbiology , nonhuman , protein content , algae , Ankistrodesmus gracilis , Eichhornia crassipes
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Inglês
Citação
Acta Scientiarum - Biological Sciences, v. 33, n. 3, p. 247-253, 2011.
