Publication: Cyclodextrin production by bacillus lehensis isolated from cassava starch: Characterisation of a novel enzyme
| dc.contributor.author | Blanco, Kate Cristina [UNESP] | |
| dc.contributor.author | De Moraes, Flávio Faria | |
| dc.contributor.author | Bernardi, Natalia Sozza [UNESP] | |
| dc.contributor.author | Vettori, Mary Helen Palmuti Braga [UNESP] | |
| dc.contributor.author | Monti, Rubens [UNESP] | |
| dc.contributor.author | Contiero, Jonas [UNESP] | |
| dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
| dc.contributor.institution | Universidade Estadual de Maringá (UEM) | |
| dc.date.accessioned | 2018-12-11T16:55:35Z | |
| dc.date.available | 2018-12-11T16:55:35Z | |
| dc.date.issued | 2014-03-04 | |
| dc.description.abstract | The properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification. Enzyme characteristics (optimum pH and temperature; pH and temperature stability), the influence of substances on the enzyme activity, enzyme kinetics, and cyclodextrin production were analysed. Cyclodextrin glycosyltransferase was purified up to 320.74-fold by affinity chromatography using β-cyclodextrin as the binder and it exhibited 8.71% activity recovery. This enzyme is a monomer with a molecular weight of 81.27 kDa, as estimated by SDS-PAGE. Optimum temperature and pH for cyclodextrin glycosyltransferase were 55°C and 8.0, respectively. The Michaelis-Menten constant was 8.62 g/l during maximum velocity of 0.858 g/l.h. | en |
| dc.description.affiliation | Department of Biochemistry and Microbiology Biological Sciences Institute UNESP-University Estadual Paulista, 1515 Bela Vista, Rio-Claro, SP | |
| dc.description.affiliation | Chemical Engineering Department UEM-University Estadual de Maringá, Maringá | |
| dc.description.affiliation | Department of Food and Nutrition Faculty of Pharmaceutical Sciences UNESP-University Estadual Paulista, Araraquara | |
| dc.description.affiliationUnesp | Department of Biochemistry and Microbiology Biological Sciences Institute UNESP-University Estadual Paulista, 1515 Bela Vista, Rio-Claro, SP | |
| dc.description.affiliationUnesp | Department of Food and Nutrition Faculty of Pharmaceutical Sciences UNESP-University Estadual Paulista, Araraquara | |
| dc.format.extent | 48-53 | |
| dc.identifier.citation | Czech Journal of Food Sciences, v. 32, n. 1, p. 48-53, 2014. | |
| dc.identifier.issn | 1212-1800 | |
| dc.identifier.scopus | 2-s2.0-84894627774 | |
| dc.identifier.uri | http://hdl.handle.net/11449/171498 | |
| dc.language.iso | eng | |
| dc.relation.ispartof | Czech Journal of Food Sciences | |
| dc.relation.ispartofsjr | 0,355 | |
| dc.rights.accessRights | Acesso restrito | |
| dc.source | Scopus | |
| dc.subject | Affinity chromatography | |
| dc.subject | Cyclodextrin glycosyltransferase | |
| dc.subject | Purification | |
| dc.title | Cyclodextrin production by bacillus lehensis isolated from cassava starch: Characterisation of a novel enzyme | en |
| dc.type | Artigo | |
| dspace.entity.type | Publication | |
| unesp.campus | Universidade Estadual Paulista (UNESP), Instituto de Biociências, Rio Claro | pt |
| unesp.department | Alimentos e Nutrição - FCF | pt |
| unesp.department | Bioquímica e Microbiologia - IB | pt |