Publicação:
In vitro cytotoxic and non-genotoxic effects of gutta-percha solvents on mouse lymphoma cells by single cell gel (comet) assay

dc.contributor.authorRibeiro, Daniel Araki [UNESP]
dc.contributor.authorMarques, Mariângela Esther Alencar [UNESP]
dc.contributor.authorSalvadori, Daisy Maria Favero [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:22:04Z
dc.date.available2014-05-27T11:22:04Z
dc.date.issued2006-12-01
dc.description.abstractChloroform and eucalyptol are widely used in clinical dentistry as gutta-percha solvents. However, these compounds may represent a hazard to human health, especially by causing injury to genetic apparatus and/or inducing cellular death. In this study, the genotoxic and cytotoxic potentials associated with exposure to chloroform and eucalyptol were assessed on mouse lymphoma cells in vitro by the single cell gel (comet) assay and trypan blue exclusion test, respectively. Both gutta-percha solvents proved to be cytotoxic at the same levels in concentrations of 2.5, 5 and 10 μL/mL (p<0.05). On the other hand, neither of the solvents induced DNA breakage. Taken together, these results suggest that although both tested compounds (chloroform and eucalyptol) are strong cytotoxicants, it seems that they are not likely to increase the level of DNA damage on mammalian cells.en
dc.description.affiliationDepartment of Pathology School of Medicine of Botucatu São Paulo State University, Botucatu, SP
dc.description.affiliationNúcleo de Avaliação Toxicogenética e Cancerígena (TOXICAN) Departamento de Patologia UNESP, Distrito de Rubião Jr. S/N, 18618-000 Botucatu, SP
dc.description.affiliationUnespDepartment of Pathology School of Medicine of Botucatu São Paulo State University, Botucatu, SP
dc.description.affiliationUnespNúcleo de Avaliação Toxicogenética e Cancerígena (TOXICAN) Departamento de Patologia UNESP, Distrito de Rubião Jr. S/N, 18618-000 Botucatu, SP
dc.format.extent228-232
dc.identifierhttp://dx.doi.org/10.1590/S0103-64402006000300010
dc.identifier.citationBrazilian Dental Journal, v. 17, n. 3, p. 228-232, 2006.
dc.identifier.doi10.1590/S0103-64402006000300010
dc.identifier.file2-s2.0-33947522051.pdf
dc.identifier.issn0103-6440
dc.identifier.issn1806-4760
dc.identifier.lattes5051118752980903
dc.identifier.lattes7528116925519142
dc.identifier.scieloS0103-64402006000300010
dc.identifier.scopus2-s2.0-33947522051
dc.identifier.urihttp://hdl.handle.net/11449/69293
dc.language.isoeng
dc.relation.ispartofBrazilian Dental Journal
dc.relation.ispartofsjr0,476
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectChloroform
dc.subjectCytotoxicity
dc.subjectEucalyptol
dc.subjectGenotoxicity
dc.subjectMouse lymphoma cells
dc.subjectchloroform
dc.subjectcineole
dc.subjectcoloring agent
dc.subjectcyclohexanol derivative
dc.subjectdiagnostic agent
dc.subjectDNA
dc.subjectgutta percha
dc.subjectmutagenic agent
dc.subjectsolvent
dc.subjectterpene
dc.subjecttrypan blue
dc.subjectanimal
dc.subjectcell survival
dc.subjectchemistry
dc.subjectcomet assay
dc.subjectDNA strand breakage
dc.subjectdrug effect
dc.subjectEucalyptus
dc.subjectleukemia L 5178 Y
dc.subjectmouse
dc.subjectpathology
dc.subjectAnimals
dc.subjectCell Survival
dc.subjectColoring Agents
dc.subjectComet Assay
dc.subjectCyclohexanols
dc.subjectDNA Breaks
dc.subjectGutta-Percha
dc.subjectLeukemia L5178
dc.subjectMice
dc.subjectMonoterpenes
dc.subjectMutagens
dc.subjectSolvents
dc.subjectTrypan Blue
dc.titleIn vitro cytotoxic and non-genotoxic effects of gutta-percha solvents on mouse lymphoma cells by single cell gel (comet) assayen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/bdj/paboutj.htm
dspace.entity.typePublication
unesp.author.lattes5051118752980903
unesp.author.lattes7528116925519142
unesp.author.orcid0000-0001-6947-5627[2]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina, Botucatupt
unesp.departmentPatologia - FMBpt

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