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Synthesis and characterization of magnetic cross-linked enzyme aggregate and its evaluation of the alternating magnetic field (AMF) effects in the catalytic activity

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dc.contributor.authorLucena, Guilherme N. [UNESP]
dc.contributor.authorSantos, Caio C. dos [UNESP]
dc.contributor.authorPinto, Gabriel C. [UNESP]
dc.contributor.authorPiazza, Rodolfo D. [UNESP]
dc.contributor.authorGuedes, Wesley N. [UNESP]
dc.contributor.authorJafelicci Júnior, Miguel [UNESP]
dc.contributor.authorde Paula, Ariela V. [UNESP]
dc.contributor.authorMarques, Rodrigo Fernando C. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-12T02:48:57Z
dc.date.available2020-12-12T02:48:57Z
dc.date.issued2020-12-15
dc.description.abstractA magnetic cross-linked enzyme aggregate (MCLEA) of cellulases and a non-magnetic analogue (CLEA) were synthesized and further investigated. The obtained MCLEA and CLEA showed a high enzyme loading efficiency of 90% and 88%, respectively. However, the MCLEA presented an activity 33% higher than the CLEA one, suggesting a catalytic enhancement effect as a result of magnetic nanoparticle presence in cross-linked cellulase aggregates structure. In addition, MCLEA shows an unusual behavior with highest enzymatic activity at low temperatures near to 37 °C. Lastly, a full factorial design (2x2) with two variables at two levels, frequency (203–420 kHz) and amplitude (3–6 kA m−1), was used in order to better investigate the effects of an alternating magnetic field (AMF) on MCLEA activity. AMF application decreased MCLEA activity, particularly in the highest values of frequency and amplitude, when compared to condition field free. In addition, frequency implied in more significant effects on MCLEA activity when compared to AMF amplitude.en
dc.description.affiliationSão Paulo State University (UNESP) Institute of Chemistry Magnetic Materials and Colloids Laboratory
dc.description.affiliationSão Paulo State University (UNESP) Institute of Chemistry Institute for Bioenergy Research (IPBEN)
dc.description.affiliationSão Paulo State University (UNESP) School of Pharmaceutical Sciences
dc.description.affiliationSão Paulo State University (UNESP) Institute of Chemistry Center for Monitoring and Research of the Quality of Fuels Biofuels Crude Oil and Derivatives (CEMPEQC)
dc.description.affiliationUnespSão Paulo State University (UNESP) Institute of Chemistry Magnetic Materials and Colloids Laboratory
dc.description.affiliationUnespSão Paulo State University (UNESP) Institute of Chemistry Institute for Bioenergy Research (IPBEN)
dc.description.affiliationUnespSão Paulo State University (UNESP) School of Pharmaceutical Sciences
dc.description.affiliationUnespSão Paulo State University (UNESP) Institute of Chemistry Center for Monitoring and Research of the Quality of Fuels Biofuels Crude Oil and Derivatives (CEMPEQC)
dc.identifierhttp://dx.doi.org/10.1016/j.jmmm.2020.167326
dc.identifier.citationJournal of Magnetism and Magnetic Materials, v. 516.
dc.identifier.doi10.1016/j.jmmm.2020.167326
dc.identifier.issn0304-8853
dc.identifier.lattes7182671143702858
dc.identifier.scopus2-s2.0-85090223651
dc.identifier.urihttp://hdl.handle.net/11449/202066
dc.language.isoeng
dc.relation.ispartofJournal of Magnetism and Magnetic Materials
dc.sourceScopus
dc.subjectAlternating magnetic field
dc.subjectEnzyme immobilization
dc.subjectMagnetic nanoparticles
dc.subjectMagnetic supports
dc.titleSynthesis and characterization of magnetic cross-linked enzyme aggregate and its evaluation of the alternating magnetic field (AMF) effects in the catalytic activityen
dc.typeArtigo
dspace.entity.typePublication
unesp.advisor.lattes7182671143702858
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Química, Araraquarapt
unesp.departmentFísico-Química - IQARpt

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Araraquara - IQAR - Instituto de Química