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Structural and functional characterization of N-terminally blocked peptides isolated from the venom of the social wasp Polybia paulista

dc.contributor.authorRibeiro, S. P.
dc.contributor.authorMendes, M. A.
dc.contributor.authordos Santos, L. D.
dc.contributor.authorde Souza, B. M.
dc.contributor.authorMarques, M. R.
dc.contributor.authorde Azevedo, W. F.
dc.contributor.authorPalma, Mario Sergio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInst Immunol Invest
dc.date.accessioned2014-05-20T13:55:38Z
dc.date.available2014-05-20T13:55:38Z
dc.date.issued2004-12-01
dc.description.abstractTwo novel peptides were isolated from the crude venom of the social wasp Polybia paulista, by using PP-HPLC under a gradient of MeCN from 5 to 60% (v/v) and named Polybine-I and -II. Further purification of these peptides under nor-mal phase chromatography rendered pure enough preparations to be sequenced by Edman degradation chemistry. However. both peptides did not interact with phenylisothiocyanate reagent, suggesting the existence of a chemically blocked N-terminus. Therefore. The sequences of both peptides were as:assigned by ESI-MS/MS under CID conditions, as follows: Polybine-I Ac-SADLVKKIWDNPA-L-NH2, (Mr 1610 Da) and Polybine-II Ac-SVDMVMKGLKIWPL-NH2 (Mr 1657 Da). During the tandem mass spectrometry experiments, a loss of 43 a.m.u. was observed from the N-terminal residue of each peptide. suggesting the acetylation of the N-terminus. Subsequently, the peptides with and without acetylation were synthesized on solid phase and submitted to functional characterizations: the biological activities investigated were: hemolysis, chemotaxis of polymorphonucleated leukocytes (PMNL), mast cell degranulation and antibiosis. The results revealed that the acetylated peptides exhibited more pronounced chemotaxis of PMNL cells and mast cell degranulation than the respective non-acetylated congeners: no hemolytic and antibiotic activities were observed, irrespective to the blockage or not of the alpha-amino groups of the N-terminal residues of each peptide. Therefore. The N-terminal acetylation may be related to the increase of the inflammatory activity of both peptides. (C) 2004 Elsevier B.V. All rights reserved.en
dc.description.affiliationUNESP, CEIS, IBRC, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationInst Immunol Invest, MCT, CNPq, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationFAPESP, CEPID, CAT, Rio Claro, SP, Brazil
dc.description.affiliationUNESP, IBILCE, Dept Phys, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespUNESP, CEIS, IBRC, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespUNESP, IBILCE, Dept Phys, Sao Jose do Rio Preto, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent2069-2078
dc.identifierhttp://dx.doi.org/10.1016/j.peptides.2004.08.019
dc.identifier.citationPeptides. New York: Elsevier B.V., v. 25, n. 12, p. 2069-2078, 2004.
dc.identifier.doi10.1016/j.peptides.2004.08.019
dc.identifier.issn0196-9781
dc.identifier.lattes2901888624506535
dc.identifier.urihttp://hdl.handle.net/11449/19916
dc.identifier.wosWOS:000226186200004
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofPeptides
dc.relation.ispartofjcr2.851
dc.relation.ispartofsjr1,001
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectPolybia paulistapt
dc.subjectN-terminally blocked peptidespt
dc.subjecttandem mass spectrometrypt
dc.subjectinflammatory peptidespt
dc.subjectwasp venom toxinspt
dc.titleStructural and functional characterization of N-terminally blocked peptides isolated from the venom of the social wasp Polybia paulistaen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dspace.entity.typePublication
unesp.author.lattes2901888624506535
unesp.author.orcid0000-0002-2078-9286[2]
unesp.author.orcid0000-0001-5832-1825[3]
unesp.author.orcid0000-0002-7363-8211[7]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Rio Claropt
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Letras e Ciências Exatas, São José do Rio Pretopt
unesp.departmentBiologia - IBpt
unesp.departmentFísica - IBILCEpt

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