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Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera

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dc.contributor.authorFerreira Junior, R. S. [UNESP]
dc.contributor.authorNascimento, N.
dc.contributor.authorMartinez, J. C. [UNESP]
dc.contributor.authorAlves, J. B.
dc.contributor.authorMeira, D. A. [UNESP]
dc.contributor.authorBarraviera, B. [UNESP]
dc.contributor.institutionCNEN
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2021-07-14T10:39:03Z
dc.date.available2021-07-14T10:39:03Z
dc.date.issued2005-09
dc.description.abstractELISA was used to evaluate, follow, and compare the humoral immune response of Swiss mice during hyperimmunization with natural and Cobalt 60-irradiated (60Co) Crotalus durissus terrificus venom. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by in vivo challenge and the side effects were assessed. The animals immunization with one LD50 of the venom was on days one, 15, 21, 30, and 45, when blood samples were collected; the challenges occurred on the 60th day. Results showed that ELISA was efficient in evaluating, following, and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those produced with irradiated venom. Immunogenic capacity was maintained after 60Co irradiation. Serum produced from Crotalus durissus terrificus irradiated venom showed higher potency and neutralization capacity than that from natural venom. All antibodies were able to neutralize five LD50 from these venoms. Clinical alterations were minimum during hyperimmunization with irradiated venom.en
dc.description.affiliationCNEN, IPEN
dc.description.affiliationUniversidade Estadual Paulista, School of Engineering
dc.description.affiliationUniversidade Estadual Paulista, Botucatu School of Medicine
dc.description.affiliationUniversidade Estadual Paulista, Center for the Study of Venoms and Venomous Animals
dc.description.affiliationUnespUniversidade Estadual Paulista, School of Engineering
dc.description.affiliationUnespUniversidade Estadual Paulista, Botucatu School of Medicine
dc.description.affiliationUnespUniversidade Estadual Paulista, Center for the Study of Venoms and Venomous Animals
dc.format.extent299-314
dc.identifierhttp://dx.doi.org/10.1590/S1678-91992005000300008
dc.identifier.citationJournal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 3, p. 299-314, 2005.
dc.identifier.doi10.1590/S1678-91992005000300008
dc.identifier.fileS1678-91992005000300008.pdf
dc.identifier.issn1678-9199
dc.identifier.scieloS1678-91992005000300008
dc.identifier.urihttp://hdl.handle.net/11449/212381
dc.language.isoeng
dc.publisherCentro de Estudos de Venenos e Animais Peçonhentos
dc.relation.ispartofJournal of Venomous Animals and Toxins including Tropical Diseases
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjecthyperimmunizationen
dc.subjectirradiationen
dc.subjectCrotalus durissus terrificusen
dc.subjectELISAen
dc.subjectserum neutralizationen
dc.subjectdetoxificationen
dc.titleImmunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antiseraen
dc.typeArtigo
dspace.entity.typePublication

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Botucatu - CEVAP - Centro de Estudos de Venenos e Animais Peçonhentos