Publicação: Polyoxyethylene 9-lauryl ether-solubilized alkaline phosphatase: Synergistic stimulation by zinc and magnesium ions
| dc.contributor.author | Ciancaglini, P. | |
| dc.contributor.author | Pizauro, J. M. | |
| dc.contributor.author | Leone, F. A. | |
| dc.contributor.institution | Universidade Estadual Paulista (UNESP) | |
| dc.date.accessioned | 2022-04-29T08:42:26Z | |
| dc.date.available | 2022-04-29T08:42:26Z | |
| dc.date.issued | 1992-01-01 | |
| dc.description.abstract | 1. 1. Polidocanol-solubilized apoalkaline phosphatase could be stimulated either by zinc ions (Kd = 8.5 nM) or by magnesium ions alone (Kd = 3.8 μM). 2. 2. Zinc and magnesium ions had synergistic effects on Polidocanol-solubilized apoalkaline phosphatase, leading to a fully active enzyme (700-800 U/mg). 3. 3. Zinc ions inhibited non-competitively the Polidocanol-solubilized apoenzyme (Ki = 7.1 μM) by displacing magnesium ions from their binding sites. 4. 4. A model for the action of zinc and magnesium ions on the modulation of the enzyme activity is proposed. © 1992. | en |
| dc.description.affiliation | Departamento de Química, Faculdade de Filosofia, Ciências e Letras, 14049 Ribeirão Preto, SP | |
| dc.description.affiliation | Departamento de Tecnologia, Faculdade Ciências Agrárias e Veteriárias, UNESP, 14870-Jaboticabal, SP, Brazil [Fax (016) 633-0567] | |
| dc.format.extent | 611-615 | |
| dc.identifier | http://dx.doi.org/10.1016/0020-711X(92)90335-X | |
| dc.identifier.citation | International Journal of Biochemistry, v. 24, n. 4, p. 611-615, 1992. | |
| dc.identifier.doi | 10.1016/0020-711X(92)90335-X | |
| dc.identifier.issn | 0020-711X | |
| dc.identifier.scopus | 2-s2.0-0026550231 | |
| dc.identifier.uri | http://hdl.handle.net/11449/230873 | |
| dc.language.iso | eng | |
| dc.relation.ispartof | International Journal of Biochemistry | |
| dc.source | Scopus | |
| dc.title | Polyoxyethylene 9-lauryl ether-solubilized alkaline phosphatase: Synergistic stimulation by zinc and magnesium ions | en |
| dc.type | Artigo | |
| dspace.entity.type | Publication | |
| unesp.department | Tecnologia - FCAV | pt |