Exploring DiPP (diisopentyl phthalate) neurotoxicity and the detoxification process in zebrafish larvae – A Silent contaminant?

Abstract

Diisopentyl phthalate (DiPP) is present in many consumer goods, but can be absorbed into the human body, and can disrupt the endocrine system affecting reproductive health and fetal development. Studies revealed that biological samples of pregnant women in Brazil contained DiPP, raising even more the concerns about its usage. This study investigated how DiPP concentrations (12.5-1000 μg l-1) affect the different developmental stages (24, 48, 72, 120, and 144 hpf) of embryos and larvae of zebrafish. DiPP induced deleterious alterations in neuromuscular development and morphometry of organs, Low concentrations of DiPP decreased acetylcholinesterase and cellular energy allocation concomitantly with increased glutathione S-transferase. Zebrafish swimming period seemed to be decreased not only due to direct neurotoxicity, but also to less allocation of energy for behavioral purposes. Moreover, high concentrations of DiPP induced spinal deformities and developmental alterations specially of the eye and liver of larvae. These findings emphasize that DiPP exerts complex effects that should be considered when assessing its potential effects on health of humans and the total environment. The biomarkers and behavioral parameters showed to be good complimentary early-warning tools exhibiting a high sensitivity compared to FET.