Publicação: Production of saccharogenic and dextrinogenic amylases by Rhizomucor pusillus A 13.36
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A newly-isolated thermophilic strain of the zygomycete fungus Rhizomucor pusillus 13.36 produced highly active dextrinogenic and saccharogenic enzymes. Cassava pulp was a good alternative substrate for amylase production. Dextrinogenic and saccharogenic amylases exhibited optimum activities at a pH of 4.0-4.5 and 5.0 respectively and at a temperature of 75°C. The enzymes were highly thermostable, with no detectable loss of saccharogenic or dextrinogenic activity after 1 h and 6 h at 60°C, respectively. The saccharogenic activity was inhibited by Ca2+ while the dextrinogenic was indifferent to this ion. Both activities were inhibited by Fe2+ and Cu2+ Hydrolysis of soluble starch by the crude enzyme yielded 66% glucose, 19.5% maltose, 7.7% maltotriose and 6.6% oligosaccharides. Copyright © 2005, The Microbiological Society of Korea.
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Amylase, Rhizomucor pusillus, Thermostability, amylase, calcium, copper, enzyme inhibitor, glucose, iron, maltose, maltotriose, oligosaccharide, starch, sucrose, trisaccharide, biosynthesis, cassava, enzyme stability, enzymology, metabolism, pH, Rhizomucor, temperature, time, Amylases, Calcium, Copper, Enzyme Inhibitors, Enzyme Stability, Glucose, Hydrogen-Ion Concentration, Iron, Maltose, Manihot, Oligosaccharides, Starch, Sucrose, Temperature, Time Factors, Trisaccharides, Fungi, Manihot esculenta
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Inglês
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Journal of Microbiology, v. 43, n. 6, p. 561-568, 2005.
