Seleção de espermatozoides para ICSI

The application of assisted reproduction techniques has provided help to many men seeking to father a child, although the current success of these procedures remains suboptimal. Today some protocols allow sperm to be selected according to their ultrastructural morphology or surface molecular characteristics. On the other hand, successful human reproduction relies partly on the inherent integrity of sperm DNA. Therefore, it is now necessary to improve the safety of the sperm selection method. It is urgent to optimize procedures to isolate spermatozoa for ICSI with low risk of DNA damage. In recent years, two technologies have attracted the attention of specialists as methods capable of identifying a spermatozoon with low risk of DNA damage: Ultrastructural morphology sperm selection at high magnification and sperm head birefringence selection. This review analyses these two technologies. © Todos os direitos reservados a SBRA - Sociedade Brasileira de Reprodução Assistida.

Keywords

Birefringence, High magnification sperm morphology, Sperm selection, double stranded DNA, povidone, acrosome, birefringence, cell nucleus, cell ultrastructure, cell vacuole, chromatin, digital imaging, DNA damage, DNA fragmentation, embryo development, fertilization in vitro, human, infertility therapy, intracytoplasmic morphologically selected sperm injection, intracytoplasmic sperm injection, male fertility, male infertility, microinjection, micromanipulation, oocyte retrieval, patient safety, pregnancy, pregnancy outcome, review, semen analysis, sperm retrieval, spermatogenesis, spermatozoon, spermatozoon abnormality, spermatozoon motility, spontaneous abortion, structure analysis