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Visual detection of turkey coronavirus RNA in tissues and feces by reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with hydroxynaphthol blue dye

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dc.contributor.authorCardoso, Tereza C. [UNESP]
dc.contributor.authorFerrari, Heitor F. [UNESP]
dc.contributor.authorBregano, Livia C. [UNESP]
dc.contributor.authorSilva-Frade, Camila [UNESP]
dc.contributor.authorRosa, Ana Carolina G. [UNESP]
dc.contributor.authorAndrade, Alexandre Lima de [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2013-09-30T19:14:53Z
dc.date.accessioned2014-05-20T13:42:39Z
dc.date.available2013-09-30T19:14:53Z
dc.date.available2014-05-20T13:42:39Z
dc.date.issued2010-12-01
dc.description.abstractA sensitive reverse-transcription loop-mediated isothermal amplification (RI-LAMP) assay was developed for the rapid visual detection of turkey coronavirus (TCoV) infection The reaction is performed in one step in a single tube at 65 degrees C for 45 min with hydroxynaphthol blue (HNB) dye added prior to amplification The detection limit of the RI-LAMP assay was approximately 10(2) EID50/50 mu l TCoV genome and no cross-reaction with other avian viruses was observed The assay was evaluated further in tissue suspensions prepared from the ileum and ileum-caecal junctions of infected turkey embryos 100% of these samples were positive in the RI-LAMP assay All individual feces samples collected in the field were considered positive by both conventional RT-PCR and RI-LAMP In conclusion RI-LAMP with HNB dye was shown to be a sensitive simple assay for the rapid diagnosis of TCoV infection either directly from feces or in association with virus isolation methods (C) 2010 Elsevier Ltd All rights reserveden
dc.description.affiliationUNESP São Paulo State Univ, Lab Anim Virol, São Paulo, Brazil
dc.description.affiliationUnespUNESP São Paulo State Univ, Lab Anim Virol, São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent415-417
dc.identifierhttp://dx.doi.org/10.1016/j.mcp.2010.08.003
dc.identifier.citationMolecular and Cellular Probes. London: Academic Press Ltd- Elsevier B.V. Ltd, v. 24, n. 6, p. 415-417, 2010.
dc.identifier.doi10.1016/j.mcp.2010.08.003
dc.identifier.issn0890-8508
dc.identifier.lattes6404176495306171
dc.identifier.urihttp://hdl.handle.net/11449/14840
dc.identifier.wosWOS:000283970200015
dc.language.isoeng
dc.publisherAcademic Press Ltd Elsevier B.V. Ltd
dc.relation.ispartofMolecular and Cellular Probes
dc.relation.ispartofjcr1.689
dc.relation.ispartofsjr0,617
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectTurkey coronavirusen
dc.subjectHydroxynaphthol blueen
dc.subjectLAMP assayen
dc.subjectSpike geneen
dc.titleVisual detection of turkey coronavirus RNA in tissues and feces by reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with hydroxynaphthol blue dyeen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderAcademic Press Ltd- Elsevier B.V. Ltd
dspace.entity.typePublication
unesp.author.lattes6404176495306171[6]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatubapt
unesp.departmentClínica, Cirurgia e Reprodução Animal - FMVApt

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Araçatuba - FMVA - Faculdade de Medicina Veterinária