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A single multiplex PCR and SNaPshot minisequencing reaction of 42 SNPs to classify admixture populations into mitochondrial DNA haplogroups

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dc.contributor.authorPaneto, Greiciane G. [UNESP]
dc.contributor.authorKoehnemann, Stephan
dc.contributor.authorMartins, Joyce A. [UNESP]
dc.contributor.authorCicarelli, Regina Maria Barretto [UNESP]
dc.contributor.authorPfeiffer, Heidi
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Munster
dc.date.accessioned2014-05-20T15:33:01Z
dc.date.available2014-05-20T15:33:01Z
dc.date.issued2011-03-01
dc.description.abstractSNaPshot minisequencing reaction is in increasing use because of its fast detection of many polymorphisms in a single assay. In this work we described a highly sensitive single nucleotide polymorphisms (SNPs) typing method with detection of 42 mitochondrial DNA (mtDNA) SNPs in a single PCR and SNaPshot multiplex reaction, in order to allow haplogroup classification in Latin American admixture population. We validated the panel typing 160 Brazilian individuals. Complete SNP profiles were obtained from 10 pg of total DNA. We conclude that it is possible to build and genotype more than forty mtDNA SNPs in a single multiplex PCR and SNaPshot reaction, with sensitivity and reliability, resolving haplogroup classification in admixture populations. (C) 2010 Elsevier B.V. and Mitochondria Research Society. All rights reserved.en
dc.description.affiliationUnesp Univ Estadual Paulista, Fac Ciencias Farmaceut, Lab Invest Paternidade, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUniv Munster, Inst Legal Med, D-48149 Munster, Germany
dc.description.affiliationUnespUnesp Univ Estadual Paulista, Fac Ciencias Farmaceut, Lab Invest Paternidade, BR-14801902 Araraquara, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipDeutscher Akademischer Austauschdienst (DAAD)
dc.description.sponsorshipIdFAPESP: 05/03984-5
dc.description.sponsorshipIdCNPq: 2007-0/140052
dc.description.sponsorshipIdDAAD: A0971734
dc.format.extent296-302
dc.identifierhttp://dx.doi.org/10.1016/j.mito.2010.12.010
dc.identifier.citationMitochondrion. Oxford: Elsevier B.V., v. 11, n. 2, p. 296-302, 2011.
dc.identifier.doi10.1016/j.mito.2010.12.010
dc.identifier.issn1567-7249
dc.identifier.urihttp://hdl.handle.net/11449/41762
dc.identifier.wosWOS:000287112100008
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMitochondrion
dc.relation.ispartofjcr3.226
dc.relation.ispartofsjr1,666
dc.rights.accessRightsAcesso restritopt
dc.sourceWeb of Science
dc.subjectAdmixture populationsen
dc.subjectBrazilen
dc.subjectHaplogroup affiliationen
dc.subjectMitochondrial DNAen
dc.subjectSNaPshot reactionen
dc.titleA single multiplex PCR and SNaPshot minisequencing reaction of 42 SNPs to classify admixture populations into mitochondrial DNA haplogroupsen
dc.typeArtigopt
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dspace.entity.typePublication
relation.isDepartmentOfPublication5004bcab-94af-4939-b980-091ae9d0a19e
relation.isDepartmentOfPublication.latestForDiscovery5004bcab-94af-4939-b980-091ae9d0a19e
relation.isOrgUnitOfPublication95697b0b-8977-4af6-88d5-c29c80b5ee92
relation.isOrgUnitOfPublication.latestForDiscovery95697b0b-8977-4af6-88d5-c29c80b5ee92
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentCiências Biológicas - FCFpt

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Araraquara - FCF - Faculdade de Ciências Farmacêuticas