Histochemical study of muscle fiber types in Synbranchus marmoratus Bloch, 1795

The myotomal muscle of Synbranchus marmoratus was investigated using histochemical and immunohistochemical reactions. This musculature is composed of a superficial red compartment, uniformly distributed around the trunk circumferentially and also in the lateral line. The red compartment fibers are small in diameter and have an oxidative metabolism, a high rate of glycogen and a negative reaction to alkaline and acid myofibrillar ATPase (mATPase). The white muscle forms the bulk of the muscle mass. Its fibers are large in diameter and have a glycolytic metabolism, a negative reaction to glycogen, a strong reaction to alkaline mATPase and a negative reaction to acid mATPase. Between these two compartments there is an intermediate layer of fibers presenting a mosaic metabolism pattern with a high rate of glycogen. These fibers stained moderately for alkaline and acid m-ATPase. Several clusters of red muscles were observed inside the white muscle. Each cluster is composed of three fiber types, with a predominance of red and intermediate fibers. Reactivity to anti-MHC BA-D5 was positive only in the intermediate fibers. Reactivity to anti-MHC SC-71 was negative in all fiber types.

Keywords

Enzyme histochemistry, Fish, Immunohistochemistry, Muscle fiber types, Synbranchus marmoratus, adenosine triphosphatase, aerobic metabolism, animal cell, fast muscle fiber, fish, glycogen metabolism, histochemistry, immunohistochemistry, muscle cell, muscle metabolism, nonhuman, slow muscle fiber, Adenosine Triphosphatases, Animals, Body Weight, Dihydrolipoamide Dehydrogenase, Fishes, Glycogen, Glycolysis, Histocytochemistry, Muscle Fibers, Muscle Fibers, Fast-Twitch, Muscle, Skeletal, Myofibrils