Logo do repositório
 

Bellis perennis extract mitigates UVA-induced keratinocyte damage: Photoprotective and immunomodulatory effects

dc.contributor.authorSouza de Carvalho, Vivian Maria
dc.contributor.authorCovre, Joyce L.
dc.contributor.authorCorreia-Silva, Rebeca D.
dc.contributor.authorLice, Izabella
dc.contributor.authorCorrêa, Mab P. [UNESP]
dc.contributor.authorLeopoldino, Andréia M.
dc.contributor.authorGil, Cristiane D. [UNESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2022-04-29T08:45:32Z
dc.date.available2022-04-29T08:45:32Z
dc.date.issued2021-08-01
dc.description.abstractA need exists for further research elucidating the benefits of environmentally safe photoprotective agents against ultraviolet (UV) exposure, and plant extracts represent a human-friendly alternative formulation. This study was designed to evaluate the potential use of Bellis perennis extract (BPE), from the Asteraceae family, known as the common daisy or the English daisy, in cosmeceuticals as a photoprotective factor, using an in vitro model of UVA-induced keratinocyte damage. Human skin keratinocytes (HaCaT cell line) were incubated with BPE at 0.01, 0.1, or 1% in Dulbecco's Modified Eagle Medium (DMEM), and after 15 min they were submitted to UVA radiation at 5, 10, and 15 J/cm2 doses, respectively. For comparative purposes, Polypodium leucotomos extract (PLE), known as the fern, was used as a positive control in assessing the photoprotective effect. After 24 h of UVA exposure, cell viability (MTT and LDH assays), levels of cleaved caspase-3, cyclooxygenase-2, IL-6, reactive oxygen species (ROS) and antioxidant enzyme (catalase, SOD, and glutathione peroxidase) activity were determined. UVA radiation at 5, 10, and 15 J/cm2 doses reduced cell viability to 63%, 43%, and 23%, respectively; we selected 10 J/cm2 for our purposes. After 24 h of UVA exposure, treatment with 1% BPE and 1% PLE significantly recovered cell viability (p < 0.05). Furthermore, treatment was associated with lower cleaved caspase-3 and ROS levels, higher catalase activity, and lower IL-6 levels in the treated UVA keratinocytes compared with the untreated UVA group (p < 0.01). Our results demonstrate photoprotective and immunomodulatory effects of BPE in skin keratinocytes and support its use as a bioactive agent in cosmetic formulations to prevent skin damage caused by exposure to the UV light.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Morfologia e Genética
dc.description.affiliationUniversidade Estadual Paulista (UNESP) Instituto de Biociências Letras e Ciências Exatas Programa de Pós-Graduação em Biociências
dc.description.affiliationUniversidade de São Paulo (USP) Faculdade de Ciências Farmacêuticas de Ribeirão Preto Departamento de Análises Clínicas Toxicológicas e Bromatológicas
dc.description.affiliationUnespUniversidade Estadual Paulista (UNESP) Instituto de Biociências Letras e Ciências Exatas Programa de Pós-Graduação em Biociências
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2017/26872-5
dc.identifierhttp://dx.doi.org/10.1016/j.jphotobiol.2021.112247
dc.identifier.citationJournal of Photochemistry and Photobiology B: Biology, v. 221.
dc.identifier.doi10.1016/j.jphotobiol.2021.112247
dc.identifier.issn1873-2682
dc.identifier.issn1011-1344
dc.identifier.scopus2-s2.0-85108512956
dc.identifier.urihttp://hdl.handle.net/11449/231464
dc.language.isoeng
dc.relation.ispartofJournal of Photochemistry and Photobiology B: Biology
dc.sourceScopus
dc.subjectAntioxidant defense
dc.subjectCleaved caspase-3
dc.subjectHaCaT
dc.subjectIL-6
dc.subjectROS
dc.subjectUVA
dc.titleBellis perennis extract mitigates UVA-induced keratinocyte damage: Photoprotective and immunomodulatory effectsen
dc.typeArtigo
dspace.entity.typePublication
unesp.departmentPrincípios Ativos Naturais e Toxicologia - FCFpt

Arquivos