Publication: Composition of constitutive heterochromatin of Pseudonannolene strinatii Mauriès, 1974 (Diplopoda, Spirostreptida) analyzed by AT/CG specific fluorochromes.
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Undergraduate course
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Abstract
Triple staining with fluorochromes (DA/DAPI/CMA) and C-banding were used to characterize the composition of Pseudonannolene strinatii heterochromatin. C-banding showed C+ bands of different labeling intensity on chromosomes 1 and 2 in some cells. Fluorochrome staining revealed DAPI+ regions corresponding to the C-banding pattern, indicating that the heterochromatin of this species is abundant in AT-rich sequences.
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Keywords
fluorescent dye, animal, arthropod, chromosome banding pattern, genetics, heterochromatin, karyotyping, methodology, Animals, Arthropods, Chromosome Banding, Fluorescent Dyes, Heterochromatin, Karyotyping
Language
English
Citation
Genetics and molecular research [electronic resource] : GMR., v. 4, n. 4, p. 765-770, 2005.
