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A label-free electrochemical affisensor for cancer marker detection: the case of HER2

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dc.contributor.authorRavalli, Andrea
dc.contributor.authorRocha, Carolina Gomes da [UNESP]
dc.contributor.authorYamanaka, Hideko [UNESP]
dc.contributor.authorMarrazza, Giovanna
dc.contributor.institutionUniversity of Florence
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-12-07T15:33:50Z
dc.date.available2015-12-07T15:33:50Z
dc.date.issued2015
dc.description.abstractIn this paper, we report the development of a sensitive label-free impedimetric biosensor based on the use of affibody as bioreceptor and gold nanostructured screen-printed graphite as a sensor platform for the detection of human epidermal growth factor receptor 2 (HER2). The affisensor is realized by immobilizing a terminal cysteine-modified affibody on gold nanoparticles. The sensor was characterized by electrochemical techniques and scanning electron microscopy (SEM). Furthermore, surface plasmon resonance (SPR) technology was also applied to explore the potential of affibodies as small-molecule discriminating tools. Using optimized experimental conditions, a single-use affisensor showed a good analytical performance for HER2 detection from 0 to 40μg/L. The estimated limit of detection was 6.0μg/L. Finally, the realized affisensor was applied to human serum samples.en
dc.description.affiliationDepartment of Chemistry Ugo Schiff, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy. Electronic address: andrea.ravalli@unifi.it.
dc.description.affiliationDepartment of Chemistry Ugo Schiff, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy; Department of Analytical Chemistry, São Paulo State University (UNESP), Rua Prof. Francisco Degni 55, 14800-060 Araraquara, SP, Brazil. Electronic address: carolgomesrocha@hotmail.com.
dc.description.affiliationDepartment of Analytical Chemistry, São Paulo State University (UNESP), Rua Prof. Francisco Degni 55, 14800-060 Araraquara, SP, Brazil. Electronic address: hidekoy@iq.unesp.br.
dc.description.affiliationDepartment of Chemistry Ugo Schiff, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy. Electronic address: giovanna.marrazza@unifi.it.
dc.description.affiliationUnespDepartment of Analytical Chemistry, São Paulo State University (UNESP), Rua Prof. Francisco Degni 55, 14800-060 Araraquara, SP, Brazil
dc.format.extent268-275
dc.identifierhttp://dx.doi.org/10.1016/j.bioelechem.2015.07.010
dc.identifier.citationBioelectrochemistry (amsterdam, Netherlands), v. 106, parte B, p. 268-275, 2015.
dc.identifier.doi10.1016/j.bioelechem.2015.07.010
dc.identifier.issn1878-562X
dc.identifier.lattes1923726000036625
dc.identifier.pubmed26260578
dc.identifier.urihttp://hdl.handle.net/11449/131320
dc.language.isoeng
dc.publisherElsevier B. V.
dc.relation.ispartofBioelectrochemistry (amsterdam, Netherlands)
dc.rights.accessRightsAcesso restritopt
dc.sourcePubMed
dc.subjectAffibodyen
dc.subjectBiosensoren
dc.subjectHer2en
dc.subjectNanoparticleen
dc.subjectTumor markeren
dc.titleA label-free electrochemical affisensor for cancer marker detection: the case of HER2en
dc.typeArtigopt
dcterms.rightsHolderElsevier B. V.
dspace.entity.typePublication
relation.isOrgUnitOfPublicationbc74a1ce-4c4c-4dad-8378-83962d76c4fd
relation.isOrgUnitOfPublication.latestForDiscoverybc74a1ce-4c4c-4dad-8378-83962d76c4fd
unesp.author.lattes1923726000036625
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Química, Araraquarapt
unesp.departmentQuímica Analítica - IQARpt

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