Histomorphometric analysis of homogenous demineralized dentin matrix as osteopromotive material in rabbit mandibles

Purpose: The aim of this work was to evaluate the effectiveness of homogenous demineralized dentin matrix (HDDM) slices in surgical bone defects created in the mandibles of rabbits and occluded with a polytetrafluoroethylene (PTFE) membrane in the promotion of bone growth. Materials and Methods: Surgical bone defects were created in 36 adult rabbits and divided into 4 groups: bone defect (control), bone defect with PTFE membrane, bone defect with HDDM, and bone defect with both HDDM and a PTFE membrane (HDDM + PTFE). The rabbits were sacrificed after 30, 60, and 90 days, and the bone defects were examined histologically and by histomorphometric analysis (analysis of variance and the Tukey test). Results: The volume of newly formed bone matrix was significantly greater in the HDDM and HDDM + PTFE groups than in the control and PTFE groups. The discrete inflammatory reaction found in the HDDM and HDDM + PTFE groups did not prevent the osteopromotive activity of the dentin matrix. Discussion: HDDM slices were biocompatible and were resorbed during the bone remodeling process. They stimulated the newly formed bone until 30 days after implantation. Conclusion: Bone repair was accelerated in the bone defects treated with HDDM in comparison to the control group.

Keywords

Bone repair, Guided tissue regeneration, Homogenous demineralized dentin matrix, Osteopromotion, Tissue engineering, anesthetic agent, antibiotic agent, diclofenac, ketamine, pentabiotic, pentabiotico, politef, rompum, unclassified drug, analysis of variance, animal experiment, animal model, animal tissue, bone demineralization, bone growth, bone matrix, bone remodeling, controlled study, dentin, evaluation, examination, histology, inflammation, jaw malformation, mandible, membrane, morphometrics, nonhuman, osteolysis, rabbit, stimulation, tooth implantation, tooth occlusion, Analysis of Variance, Animals, Biocompatible Materials, Bone Matrix, Bone Remodeling, Bone Substitutes, Dental Materials, Dentin, Guided Tissue Regeneration, Haversian System, Mandibular Diseases, Membranes, Artificial, Osteogenesis, Polytetrafluoroethylene, Rabbits, Time Factors