São José dos Campos - ICT - Instituto de Ciência e Tecnologia

URI Permanente para esta coleçãohttps://hdl.handle.net/11449/253778

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Resveratrol-coated gold nanorods produced by green synthesis with activity against Candida albicans
(2024-10-22) Carmo, Paulo Henrique Fonseca do ; Lage, Anna Carolina Pinheiro ; Garcia, Maíra Terra ; Silva, Newton Soares da ; Santos, Daniel Assis ; Mylonakis, Eleftherios ; Junqueira, Juliana Campos ; Universidade Estadual Paulista (Unesp)
Candida albicans is an opportunistic yeast capable of causing a wide range of mucosal, cutaneous, and systemic infections. However, therapeutic strategies are limited to a few antifungal agents. Inorganic nanoparticles have been investigated as carrier systems for antifungals as potential new treatments. In this study, we focused on the antifungal activity of gold nanorods, a specific rod-shaped gold nanoparticle, produced by green synthesis using resveratrol as a metal-reducing agent. The synthesis method resulted in stable control nanoparticles (AuNp) and resveratrol-coated gold nanoparticles (AuNpRSV) with medium sizes of 32.4 × 15.9 nm for AuNp, and 33.5 × 15.3 nm for AuNpRSV. Both AuNp and AuNpRSV inhibited the C. albicans grown at 2.46 μg/mL, exhibited fungicidal effects at 4.92 μg/mL, and significantly decreased filamentation, biofilm viability, reactive oxygen species production and ergosterol levels of C. albicans. In addition, exposure to AuNpRSV reduced the ability of C. albicans to grow in the presence of cell membrane stressors. Transmission electron microscopy revealed enlargement of the cell wall and retraction of the cell membrane after treatment with AuNp and AuNpRSV. Promisingly, in vivo toxicity analysis demonstrated that both nanoparticles maintained the full viability of Galleria mellonella larvae at 49.20 μg/mL. In conclusion, both gold nanoparticles exhibited antifungal activity; however, these effects were enhanced by AuNpRSV. Altogether, AuNps and AuNpRSVs are potential antifungal agents for the treatment of C. albicans infections.
An 8-year follow-up of resin infiltration on anterior white spot lesions
(2023-01-01) Omoto, Érika M ; Oliveira, Laryssa C. ; Rocha, Rafael S. ; Machado, Lucas Silveira ; Bresciani, Eduardo ; Prakki, Anuradha ; Fagundes, Ticiane Cestari ; Universidade Estadual Paulista (UNESP) ; Federal University of Rio Grande do Sul ; University of Toronto
White spot lesions (WSLs) are sites of enamel surface and subsurface demineralization that increases tissue porosity and affects the teeth appearance. The resin infiltration technique proved to be a valid alternative to arrest caries lesion progression and to masking a color change in noncavitated WSLs. Thus, this study aims to report a clinical case of anterior WSLs treated with resin infiltration technique with an 8-year follow-up. The resin infiltration protocol was performed in an 18-year-old female patient presenting WSLs on the maxillary right lateral incisor, left central incisor, and left canine. The protocol followed the manufacturer's recommendations. The patient reported satisfaction with the smile appearance, at the end of the appointment. Infiltrated areas remained unchanged after an 8-year follow-up, showing an acceptable result for the patient's esthetic desires. After 8 years of evaluation, the resin infiltration technique proved to be a resistant and reliable alternative in preventing caries progression and in color masking WSLs.
β-TCP/S53P4 Scaffolds Obtained by Gel Casting: Synthesis, Properties, and Biomedical Applications
(2023-05-01) Amaral, Suelen Simões ; Lima, Beatriz Samara de Sousa ; Avelino, Sarah Oliveira Marco ; Spirandeli, Bruno Roberto ; Campos, Tiago Moreira Bastos ; Thim, Gilmar Patrocínio ; Trichês, Eliandra de Sousa ; Prado, Renata Falchete do ; Vasconcellos, Luana Marotta Reis de ; Universidade Estadual Paulista (UNESP) ; Universidade de São Paulo (USP) ; Technological Institute of Aeronautics (ITA)
The objective of this study was to investigate the osteogenic and antimicrobial effect of bioactive glass S53P4 incorporated into β-tricalcium phosphate (β-TCP) scaffolds in vitro and the bone neoformation in vivo. β-TCP and β-TCP/S53P4 scaffolds were prepared by the gel casting method. Samples were morphologically and physically characterized through X-ray diffraction (XRD) and scanning electron microscope (SEM). In vitro tests were performed using MG63 cells. American Type Culture Collection reference strains were used to determine the scaffold’s antimicrobial potential. Defects were created in the tibia of New Zealand rabbits and filled with experimental scaffolds. The incorporation of S53P4 bioglass promotes significant changes in the crystalline phases formed and in the morphology of the surface of the scaffolds. The β-TCP/S53P4 scaffolds did not demonstrate an in vitro cytotoxic effect, presented similar alkaline phosphatase activity, and induced a significantly higher protein amount when compared to β-TCP. The expression of Itg β1 in the β-TCP scaffold was higher than in the β-TCP/S53P4, and there was higher expression of Col-1 in the β-TCP/S53P4 group. Higher bone formation and antimicrobial activity were observed in the β-TCP/S53P4 group. The results confirm the osteogenic capacity of β-TCP ceramics and suggest that, after bioactive glass S53P4 incorporation, it can prevent microbial infections, demonstrating to be an excellent biomaterial for application in bone tissue engineering.
Development of Y-TZP/MWCNT-SiO2 nanocomposite for dental protheses
(2023-07-01) da Silva, Lucas Hian ; Rinaldi, Laura Ajamil ; Lazar, Dolores Ribeiro Ricci ; Ussui, Valter ; Tango, Rubens Nisie ; Belli, Renan ; Lohbauer, Ulrich ; Cesar, Paulo Francisco ; Universidade de São Paulo (USP) ; Nuclear and Energy Research Institute (IPEN) ; Universidade Estadual Paulista (UNESP) ; Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
Y-TZP/MWCNT-SiO2 nanocomposite was synthesized by co-precipitation and hydrothermal treatment methods. After the characterization of the MWCNT-SiO2 powder, specimens were obtained from the synthesized material Y-TZP/MWCNT-SiO2 by uniaxial pressing for a second characterization and later comparison of its optical and mechanical properties with the conventional Y-TZP. The MWCNT-SiO2 was presented in bundles of carbon nanotubes coated by silica (mean length: 5.10 ± 1.34 μm /D90: 6.9 μm). The composite manufactured was opaque (contrast ratio: 0.9929 ± 0.0012) and had a white color with a slightly difference from the conventional Y-TZP (ΔE00: 4.4 ± 2.2) color. The mechanical properties of Y-TZP/MWCNT-SiO2: vickers hardness (10.14 ± 1.27 GPa; p = 0.25) and fracture toughness (4.98 ± 0.30 MPa m1/2; p = 0.39), showed no significant difference from the conventional Y-TZP (hardness: 8.87 ± 0.89; fracture toughness: 4.98 ± 0.30 MPa m1/2). However, for flexural strength (p = 0.003), a lower value was obtained for Y-TZP/MWCNT-SiO2 (299.4 ± 30.5 MPa) when compared to the control Y-TZP (623.7 ± 108.8 MPa). The manufactured Y-TZP/MWCNT-SiO2 composite presented satisfactory optical properties, however the co-precipitation and hydrothermal treatment methods need to be optimized to avoid the formation of porosities and strong agglomerates, both from Y-TZP particles and MWCNT-SiO2 bundles, which lead to a significant decrease in the material flexural strength.
Sem título
(2023-04-01) Silveira, Marcos Paulo Motta ; Ramos, Nathália de Carvalho ; Lopes, Guilherme da Rocha Scalzer ; Tribst, João Paulo Mendes ; Bottino, Marco Antonio ; Universidade Estadual Paulista (UNESP) ; University of Taubaté (UNITAU) ; The University of Amsterdam and Vrije Universiteit
Error in Figure In the original publication [1], there was a mistake in Figure 2 as published. “Aging” labels referring to “Yes” and “No” on the figure were switched. The corrected Figure 2 appears below. The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.
Enhancing biocompatibility and bone neoformation with nanostructured calcium aluminate cement
(2023-06-01) dos Santos, Andrea Fernanda Lopes ; Vieira, Paula Fonseca Antunes ; de Araújo, Juliani Caroline Ribeiro ; de Vasconcellos, Luana Marotta Reis ; Castilho, Maiara Lima ; de Oliveira, Ivone Regina ; Raniero, Leandro ; University of Vale Do Paraíba ; Universidade Estadual Paulista (UNESP)
Purpose: Because of bone loss, bone complex fractures require medical intervention. Bone grafting may result in a positive recovery from bone loss. As a result, attempts are being made to investigate new bone grafting materials as alternatives, as well as to reproduce bone specificities on a wide scale. Because of its characteristics, Homogeneous Calcium Aluminate Cement (CACH) is a viable candidate for bone substitution. Gold (AuNPs) and silver (AgNPs) nanoparticles can improve CACH efficiency. The biocompatibility of CACH material linked with AuNPs and AgNPs was examined in vitro and in vivo in this study. Methods: SEM and mitochondrial activity percentage fluctuation were used to analyze materials in vitro for cell adhesion, proliferation, and biocompatibility. Meanwhile, histological examination of samples in vivo searched for bone neoformation. Results: SEM and mitochondrial activity percentage variation revealed efficient cell adhesion and proliferation when linking biocompatible material. In both samples, histological investigation revealed bone neoformation. CACH linked with AuNPs, on the other hand, produced the most relevant results. Conclusion: Although both samples showed bone neoformation, CACH combined with AuNPs generated a potentially efficient bone repair material as an enhanced bone substitute.
Parents with periodontitis drive the early acquisition of dysbiotic microbiomes in their offspring
(2023-07-01) Reis, Aurélio Amorim ; Monteiro, Mabelle Freitas ; Bonilha, Gabriela Martin ; Saraiva, Luciana ; Araújo, Cassia ; Santamaria, Mauro Pedrine ; Casati, Marcio Zaffalon ; Kumar, Purnima ; Casarin, Renato Corrêa Viana ; Universidade Estadual de Campinas (UNICAMP) ; Universidade de São Paulo (USP) ; Universidade Estadual Paulista (UNESP) ; University of Michigan
Aim: To evaluate the microbial colonization in different dentition phases on individuals from 0 to 18 years of age belonging to families with a history of periodontitis compared to descendants of periodontally healthy parents. Materials and Methods: The offspring of subjects with periodontitis (‘Perio’ group) and the offspring of periodontally healthy subjects (‘Healthy’ group), matched for gender and age, were included in this cross-sectional study and divided according to the dentition phase: pre-dentate, primary, mixed and permanent. The patients were clinically assessed, and their saliva was collected. DNA was extracted, and V1–V3 and V4–V5 regions of the 16S rRNA gene were sequenced. Results: Fifty children of parents with periodontitis and 50 from healthy parents were included in the study and divided according to the dentition phase: pre-dentate (n = 5/group), primary dentition (n = 15/group), mixed dentition (n = 15/group) and permanent dentition (n = 15/group) in each group. The microbiome composition was different between dentitions for both groups. Children of the Perio group presented a microbial diversity different from that of the Healthy group in mixed and permanent dentitions. The more intense shift in the community occurred between primary and mixed dentition in the Perio group, while the transition between mixed and permanent dentition was the period with greater changes in the microbiome for the Healthy group. Furthermore, a pathogen-rich environment—higher prevalence and abundance of periodontitis-associated species such as Prevotella spp., Selenomonas spp., Leptotrichia spp., Filifactor alocis, Prevotella intermedia, Treponema denticola and Tannerella forsythia— was observed in the Perio group. Conclusions: The parents' periodontal status significantly affects the microbiome composition of their offspring from an early age. The mixed dentition was the phase associated with establishing a dysbiotic and pathogen-rich microbiome in descendants of parents with periodontitis.
Cone-beam computed tomography texture analysis can help differentiate odontogenic and non-odontogenic maxillary sinusitis
(2023-01-01) Costa, Andre Luiz Ferreira ; Fardim, Karolina Aparecida Castilho ; Ribeiro, Isabela Teixeira ; Jardini, Maria Aparecida Neves ; Braz-Silva, Paulo Henrique ; Orhan, Kaan ; de Castro Lopes, Sérgio Lúcio Pereira ; Cruzeiro do Sul University ; Universidade Estadual Paulista (UNESP) ; Universidade de São Paulo (USP) ; Ankara University
Purpose: This study aimed to assess texture analysis (TA) of cone-beam computed tomography (CBCT) images as a quantitative tool for the differential diagnosis of odontogenic and non-odontogenic maxillary sinusitis (OS and NOS, respectively). Materials and Methods: CBCT images of 40 patients diagnosed with OS (N=20) and NOS (N=20) were evaluated. The gray level co-occurrence (GLCM) matrix parameters, and gray level run length matrix texture (GLRLM) parameters were extracted using manually placed regions of interest on lesion images. Seven texture parameters were calculated using GLCM and 4 parameters using GLRLM. The Mann-Whitney test was used for comparisons between the groups, and the Levene test was performed to confirm the homogeneity of variance (α=5%). Results: The results showed statistically significant differences (P<0.05) between the OS and NOS patients regarding 3 TA parameters. NOS patients presented higher values for contrast, while OS patients presented higher values for correlation and inverse difference moment. Greater textural homogeneity was observed in the OS patients than in the NOS patients, with statistically significant differences in standard deviations between the groups for correlation, sum of squares, sum of entropy, and entropy. Conclusion: TA enabled quantitative differentiation between OS and NOS on CBCT images by using the parameters of contrast, correlation, and inverse difference moment.
Stress distribution on implant- supported zirconia crown of maxillary first molar: effect of oblique load on natural and antagonist tooth
(2023-01-01) Leandro, Lisliane Nara Rossi ; Barra Grande, Mateus Favero ; Pelegrine, André Antônio ; Nishioka, Renato Sussumu ; Teixeira, Marcelo Lucchesi ; Basting, Roberta Tarkany ; Faculdade São Leopoldo Mandic ; Universidade Estadual Paulista (UNESP)
This study evaluated the stress distribution on an implant-supported zirconia crown of a mandibular first molar subjected to oblique loading by occlusal contact with the natural maxillary first molar by using the 3D finite element method. Two virtual models were made to simulate the following situations: (1) occlusion between maxillary and mandibular natural first molars; (2) occlusion between zirconia implant-supported ceramic crown on a mandibular first molar and maxillary natural first molar. The models were designed virtually in a modeling program or CAD (Computer Aided Design) (Rhinoceros). An oblique load of 100 N was uniformly applied to the zirconia framework of the crown. The results were obtained by the Von Mises criterion of stress distribution. Replacement of the mandibular tooth by an implant caused a slight increase in stress on portions of the maxillary tooth roots. The crown of the maxillary model in occlusion with natural antagonist tooth showed 12% less stress when compared with the maxillary (model in occlusion with the) implant-supported crown. The mandibular crown of the implant show 35% more stress when compared with the mandibular antagonist crown on the natural tooth. The presence of the implant to replace the mandibular tooth increased the stresses on the maxillary tooth, especially in the region of the mesial and distal buccal roots.
Antifungal Action of Herbal Plants’ Glycolic Extracts against Candida Species
(2023-03-01) Meccatti, Vanessa Marques ; Santos, Lana Ferreira ; de Carvalho, Lara Steffany ; Souza, Clara Bulhões ; Carvalho, Cláudio Antonio Talge ; Marcucci, Maria Cristina ; Abu Hasna, Amjad ; de Oliveira, Luciane Dias ; Universidade Estadual Paulista (UNESP)
Candida spp. cause fungal infection that affects patients’ oral health. This study aimed to evaluate the isolated and synergistic antifungal effect of Rosa centifolia L., Curcuma longa L., Rosmarinus officinalis L., and Punica granatum L. glycolic extracts against Candida albicans, Candida dubliniensis, Candida tropicalis, and Candida krusei planktonic and biofilm forms. The plant extracts were chemically characterized and the main compounds were quantified by high-performance liquid chromatography (HPLC-DAD) analysis. The minimum inhibitory and minimum fungicidal concentrations of the extracts were determined, and antibiofilm activity was evaluated by MTT assay. Data were analyzed by one-way ANOVA and Tukey’s tests, and by Kruskal–Wallis and Dunn’s tests, considering a significance level of 5%. The main compounds identified in each of the extracts were: p-coumaric acid (2153.22 μg/100 mL) in the rosemary extract, gallotannins (4318.31 μg/100 mL) in the pomegranate extract, quercetin derivatives (3316.50 μg/100 mL) in the extract of white roses, and curcumin (135.09 μg/100 mL) in the turmeric extract. The combination of R. centifolia and C. longa glycolic extracts was effective against C. albicans, C. dubliniensis, and C. tropicalis biofilms over different periods (p < 0.05). The combination of R. officinalis and P. granatum glycolic extracts was effective against C. albicans and C. krusei biofilms after 30 min, and against C. tropicalis after 24 h, with all combinations showing an average reduction of 50% in cell viability (p < 0.05). In conclusion, the combined plant extracts have antifungal and antibiofilm action against Candida spp. in different concentrations and times of action.
Zn:DLC films via PECVD-HIPIMS: Evaluation of antimicrobial activity and cytotoxicity to mammalian cells
(2023-05-01) De O. Correia, Rebeca F.B. ; Sampaio, Aline G. ; Milhan, Noala V.M. ; Capote, Ariel ; Gerdes, Holger ; Lachmann, Kristina ; Trava-Airoldi, Vladimir J. ; Koga-Ito, Cristiane Yumi ; Bandorf, Ralf ; National Institute for Space Research - INPE ; Universidade de São Paulo (USP) ; Fraunhofer Institute for Surface Engineering and Thin Films (IST) ; Universidade Estadual Paulista (UNESP)
DLC films were grown with Zn via a combined plasma-enhanced chemical vapor deposition (PECVD) and high-power impulse magnetron sputtering (HIPIMS) process. The films were deposited on textiles in an atmosphere of Ar and C2H2, and the percentage of metal in the DLC was varied by controlling the acetylene gas flow. At first, to evaluate the antimicrobial activity, a screening test with the ISO 22196 standard was carried out. Afterward, AATCC TM100:2019 was used to evaluate the antimicrobial effectiveness of the films on textiles. The antimicrobial effectiveness of the coating was studied against a Gram-negative bacterium (Escherichia coli), a Gram-positive bacterium (Staphylococcus aureus), and a fungus (Candida albicans), after a 24 h contact. In addition, the cytotoxicity of the samples to mammalian cells was evaluated by indirect contact. For this, the samples were soaked into the growth media for 1 and 7 days, and then, the extracts were collected and put in contact with keratinocytes for 24 h. Finally, the properties of the films were also evaluated as a function of the Zn content, such as their structural quality, morphology, hardness, wear resistance, and coefficient of friction. The films showed excellent results against all microorganisms, with 100% effectiveness in some cases. The pure extracts obtained from all the samples with the incorporation of metals were cytotoxic. Despite that, the cell viability after contact with some Zn-DLC diluted extracts (10%) was not different from that observed in the uncoated group. Besides, increasing the Zn content resulted in a film with poorer mechanical properties but did not affect the coefficient of friction of the coating.
Hidden colon adenocarcinoma diagnosed from mouth metastasis: case report and literature review
(2023-12-01) de Almeida Lança, Maria Leticia ; Carvalho, Yasmin Rodarte ; Almeida, Janete Dias ; Kaminagakura, Estela ; Universidade Estadual Paulista (UNESP)
Background: We report an unusual case of metastatic colon adenocarcinoma to the maxilla as an initial clinical sign of the disease, this being the second case reported in the palate. In addition, we show an extensive review of the literature, with clinical cases of adenocarcinoma with metastasis to the mouth. Case presentation: An 80-year-old man complained of “swelling on the palate” with a 3-week evolution time. He reported suffering from constipation and high blood pressure. The intraoral examination revealed a pedunculated, red, and painless nodule on the maxillary gingiva. Under the diagnostic hypotheses of squamous cell carcinoma and malignant neoplasm of the salivary gland, an incisional biopsy was performed. Microscopically, the columnar epithelium was observed forming papillary areas, neoplastic cells with prominent nucleoli, hyperchromatic nuclei, atypical mitotic figures, and mucous cells, being positive for CK 20, suggesting the provisional diagnosis of metastatic adenocarcinoma, probably of gastrointestinal origin. The patient was submitted to endoscopy and colonoscopy exams, and a lesion in the sigmoid region of the colon was observed. After a colon biopsy, a moderately differentiated adenocarcinoma was confirmed, establishing the final diagnosis of metastatic neoplasia of colon adenocarcinoma to the oral lesion. The literature review revealed 45 clinical cases of colon adenocarcinoma with metastasis to the oral cavity. To the best of our knowledge, it is the second case on the palate. Conclusions: Colon adenocarcinoma with metastasis to the oral cavity is rare but should be included in the differential diagnosis of neoplasms of the oral cavity, even when there are no known primary tumors in some cases, and this may be the first indication of the presence of a tumor.
Fatigue survival of endodontically treated teeth restored with different fiber-reinforced composite resin post strategies versus universal 2-piece fiber post system: An in vitro study
(2023-03-01) Penteado, Marcela Moreira ; de Andrade, Guilherme Schmitt ; Araujo, Rodrigo Máximo ; Borges, Alexandre Luiz Souto ; Valandro, Luiz Felipe ; Pereira, Gabriel Kalil Rocha ; da Silva, João Maurício Ferraz ; Universidade Estadual Paulista (UNESP) ; Universidade Federal de Sergipe (UFS)
Statement of problem: Various strategies have been proposed to reduce the cement space of foundation restorations for endodontically treated teeth. However, they may add more operative steps, or the dentist must keep different sizes of drills and posts in stock. A 2-piece universal adjustable post system has been developed to overcome this problem, but whether the system has acceptable fatigue survival performance is unclear. Purpose: The purpose of this in vitro study was to evaluate the fatigue survival and stress distribution of endodontically treated teeth without a ferrule and restored with different glass fiber post strategies versus a recently introduced universal 2-piece fiber post system. Material and methods: Bovine incisor roots were randomly assigned to 3 groups as per the post used (n=13): adapted glass fiber post with post space preparation of the same size, composite resin-custom glass fiber post (CTM), and universal 2-piece glass fiber-reinforced composite resin post (UNI). The posts were adhesively luted, the composite resin core was added, and a composite resin crown was produced with computer-aided design and computer-aided manufacturing (CAD-CAM), and then adhesively luted to each core. A fatigue test was performed with the stepwise stress method (10 000 cycles/step; 20 Hz; load=100 N to 750 N; step=50 N) until fracture, and the failure mode analyzed. The stress distribution was evaluated by finite element analysis with the maximum principal stress criteria by following the parameters of the in vitro test. The solids were considered homogeneous, linear, and isotropic, except for the glass fiber post (orthotropic), and a load of 450 N at 30 degrees was applied. The fatigue failure load and the number of cycles for failure were analyzed with Kaplan-Meier and Mantel-Cox (log rank test) (α=.05). The finite element analysis results were analyzed with colorimetric graphs. Results: The highest fatigue failure load and the number of cycles for failure were found in the UNI system, whereas the lowest results were found in the CTM group. All groups exhibited repairable failures. The finite element analysis showed the lowest stress in root dentin in the UNI system. The CTM system had the largest stress regions at the dentin and dentin-core interface. Conclusions: The use of a 2-piece universal glass fiber post system resulted in more fatigue behavior compared with composite resin-custom glass fiber posts.
EFFECT OF SURFACE TREATMENT ON FLEXURAL STRENGTH AND SUBCRITICAL CRACK GROWTH OF LITHIUM DISILICATE: AN IN VITRO STUDY
(2023-01-20) DE CARVALHO, Ronaldo Luís Almeida ; Miranda, Jean Soares ; Ramos, Nathália de Carvalho ; Weitzel, Isabela Sandim Souza Leite ; Amaral, Marina ; Kimpara, Estevão Tomomitsu ; Centro Universitário Braz Cubas ; Universidade Federal de Juiz de Fora ; Universidade São Francisco: Bragança Paulista ; Universidade de Taubate ; Universidade Estadual Paulista (UNESP)
Alternative surface treatments have been proposed for the cementation of lithium disilicate ceramics aiming to improve adhesive and flexural strength under fatigue. This study aimed to evaluate the slow crack growth (SCG) parameters of the lithium disilicate ceramic after hydrofluoric acid (HF) etching or air abrasion (AB) as surface treatments. Ceramic discs were treated with HF (5%, 20 s) or AB (30 µm silica-modified alumina particles, 2.8 bar, 10 mm distance, 15 s), and received a layer of resin cement. The surface roughness after surface treatment was evaluated (n = 5). Samples were tested in a piston-on-three-ball assembly to evaluate the flexural strength (n = 20), inert strength (n = 25), and to determine SCG parameters n and D (n = 35). The highest roughness (p < 0.01) was observed in the AB group, with the highest reliability according to the Weibull analysis, but the lowest SCG susceptibility. Flexural (p = 0.03) and inert strength (p < 0.01) were the greatest in the HF group. Despite exhibiting lower strength than 5% HF, air abrasion may be an alternative for the surface treatment of lithium disilicate surfaces, indicating the best prognosis over time.
Fluoride and Calcium Release from Alkasite and Glass Ionomer Restorative Dental Materials: In Vitro Study
(2023-02-01) Di Lauro, Alessandro ; Di Duca, Fabiana ; Montuori, Paolo ; Dal Piva, Amanda Maria de Oliveira ; Tribst, João Paulo Mendes ; Borges, Alexandre Luiz Souto ; Ausiello, Pietro ; University of Naples Federico II ; University of Amsterdam and Vrije Universiteit Amsterdam ; Universiteit van Amsterdam and Vrije Universiteit Amsterdam ; Universidade Estadual Paulista (UNESP)
This study evaluated the effect of pH and temperature on the ion (F− and Ca2+) release of a resin-based material containing alkaline fillers and a self-setting high-viscous glass ionomer cement. Disks were prepared according to manufacturers’ instructions for both materials: the EF group (Equia Forte HT filling, GC) and the CN group (Cention N, Ivoclar). Specimens were immersed in 50 mL buffer solution with three different pHs (4.8, 6.8, and 8.8), and stored at 0°, 18°, 37°, and 44 °C. After 24 h, 7 d, and 28 d, cumulative F− and Ca2+ releases were analyzed by chromatography and mass spectrometry, and pH was measured. Both materials showed minimal changes in pH with final values after 28 d of 5.17 ± 0.56 for CN and 5.12 ± 0.24 for EF. In all experimental conditions, the percentages of ion release were higher for EF than for CF. In particular, both materials showed a significant difference in temperature in F− release. Regardless of the pH values, the highest Ca2+ ion release was after 28 days, with a significant difference in temperature for CN and EF. Within the limit of this study, the temperature storage influenced ion release and the high-viscous glass ionomer showed the maximum values.
Surface smoothness of resin composites after polishing—A systematic review and network meta-analysis of in vitro studies
(2023-04-01) de Oliveira, Ananda Gomes ; Rocha, Rafael Santos ; Spinola, Manuela da Silva ; Batista, Graziela Ribeiro ; Bresciani, Eduardo ; Caneppele, Taciana Marco Ferraz ; Universidade Estadual Paulista (UNESP) ; Braz Cubas University ; A. T. Still University
A systematic review and network meta-analysis was performed to provide evidence for the best polishing protocol for different types of resin composites to minimize surface roughness. A search was performed in PubMed, Scopus, Web of Science, LILACS, BBO, EMBASE, and Cochrane Library on July 2, 2019 (updated in December, 2020). In vitro studies that included at least two systems for polishing resin composites and analyzed surface roughness were included. The risk of bias was evaluated. A random-effects Bayesian-mixed treatment comparison model was used to compare surface roughness in resin composites with the different types of polishers. Surface under the cumulative ranking curve (SUCRA) analysis was performed to rank the probability for the best polishing system. After removal of duplicates, title and abstract screening yielded 34 studies. Network meta-analysis was not possible for hybrid and microhybrid composites. SUCRA analysis showed that abrasive paper discs allowed greater surface smoothness for nanohybrid and nanofill composites to a probability of between 83% and 91.6%. Silicon carbide brush had a 78.2% probability of being the best system for microfill composites. The use of abrasive paper disc polishers showed a favorable result in nanofill and nanohybrid resin composites. Silicon carbide brush has a greater chance of promoting a smoother surface for microfill resin composites.
Effect of Photobiomodulation Therapy Associated With Biphasic Phosphate Calcium on Bone Repair: A Histomorphometric Study in Rats
(2022-01-01) De Marco, Andréa Carvalho ; Torquato, Letícia Cavassini ; Ribeiro, Tatiana Camacho ; Nunes, Camilla Moretto ; Bernardo, Daniella Vicensotto ; Maciel, Clarissa Carvalho Martins ; Pereira, Kauê Alberto ; Jardini, Maria Aparecida Neves ; Santamaria, Mauro Pedrine ; Universidade Estadual Paulista (UNESP) ; Faculdade Santo Antônio
Introduction: This study aimed to investigate the effects of photobiomodulation (PBM) therapy associated with biphasic calcium phosphate on calvaria critical defects in rats. Methods: Forty-eight (90 days old) adult male rats (Rattus norvegicus, Albinus variation, Wistar) received critical defects of 5 mm in diameter, which were made on their skull, and they were randomly assigned into the following groups: C-blood clot, B-biphasic calcium phosphate, L-photobiomodulation therapy, and B + L-biphasic calcium phosphate + photobiomodulation therapy. A low-level a gallium aluminum arsenide (GaAlAs) laser was applied in a single dose during surgery, in a wavelength of 660 nm and total energy density of 45 J/cm2. On 30th and 60th days, the animals from each group were euthanized. Histological and histomorphometric analyses were performed. Results: In 30 days, almost all specimens (C, L, B and B + L) showed bone neoformation areas in regions near the borders of the surgical defect. In 60 days, in many specimens (C, L, B, B + L), it was possible to see a narrow neoformed bone structure along almost the whole extension of the surgical defect, though it was thinner than the original calvary bone. Data were recorded as mean ± standard deviation, and after normality was tested, a suitable statistical test was applied (α = 5%). On day 60, there was a statistically significant difference when comparing the proportion of neoformation area between group L (0.52% ± 0.13) and group B + L (0.20% ± 0.08). Group L showed a difference compared with all the groups when we compared the remaining distance between the edges of neoformed bone (C × L, P = 0.0431; B × L, P = 0.0386; L × B + L, P = 0.0352), demonstrating a great defect closure. Conclusion: Our findings suggest that although biphasic calcium phosphate exerts some osteogenic activity during bone repair, PBM therapy is not able to modulate this process
Effects of lesion size on the 30-month clinical performance of restorations with bulk fill and a regular nanofilled resin composite in noncarious cervical lesions
(2023-01-01) Correia, Ayla Macyelle de Oliveira ; Jurema, Ana Luiza Barbosa ; Bresciani, Eduardo ; Caneppele, Taciana Marco Ferraz ; Universidade Estadual Paulista (UNESP)
Objective: The aim of this double-blind, randomized trial was to evaluate the influence of the occlusogingival distance (OGD) in noncarious cervical lesions (NCCLs) on the clinical performance of a regular bulk-fill resin composite and a regular nanofilled resin composite. Materials and methods: One hundred and forty NCCLs in 77 participants were randomly divided into four groups (n = 35), according to OGD (1.5 mm ± 10% or 3 mm ± 10%) and resin composites (Filtek Bulk Fill Posterior [B] or Filtek Z350 XT [C]), namely: 1.5 mm-B, 1.5 mm-C, 3 mm-B, and 3 mm-C. The restorations were bonded using a two-step self-etch adhesive (Clearfil SE Bond), applied following the manufacturer’s instructions. Restorations were polished 1 week after placement. Two experienced and calibrated examiners evaluated the restorations using modified US Public Health Service criteria at baseline (7 days) and after 6, 12, 18, 24, and 30 months. Statistical analyses were carried out using Kruskal–Wallis, Friedman’s repeated measures analysis of variance, and the Wilcoxon signed rank test (α = 0.05). Results: After 30 months, the recall rate was 94.2%. Eight restorations were lost (3 for 1.5 mm-C, 2 for 1.5 mm-B, 1 for 3 mm-C, and 2 for 3 mm-B). All groups resulted in a significantly worse marginal discoloration and surface texture at 30 months in comparison with the baseline (1 week). No significant difference was found for the other parameters. Conclusion: The restorations performed with both resin composites produced clinically acceptable restorations. The OGD of NCCLs did not influence the clinical performance of restorations. Clinical significance: The OGD of NCCLs did not have a significant effect on the clinical performance of a regular bulk-fill and nanofilled restorations. Both materials showed a very good performance on 30-month evaluation.
Multiple gingival recessions associated with non-carious cervical lesions treated by partial restoration and modified coronally advanced flap with either connective tissue graft or xenogeneic acellular dermal matrix: A randomized clinical trial
(2023-01-01) Santamaria, Mauro Pedrine ; Rossato, Amanda ; Fernanda Ferreira Ferraz, Laís ; Maria Viana Miguel, Manuela ; Venturoso Simões, Beatriz ; Pereira Nunes, Marcelo ; Bresciani, Eduardo ; de Sanctis, Massimo ; Mathias-Santamaria, Ingrid Fernandes ; Universidade Estadual Paulista (UNESP) ; College of Dentistry ; São Paulo ; Università Vita-Salute San Raffaele ; University of Maryland School of Dentistry
Background: This study aimed to compare a connective tissue graft (CTG) to a (porcine) xenogeneic acellular dermal matrix (XDM), both associated with modified coronally advanced flap (MCAF) and partial resin composite restoration to treat multiple combined defects (CDs). Methods: Seventy-eight defects in 38 patients presenting multiple combined defects, that is, gingival recession associated with non-carious cervical lesions, were treated by partial resin composite restoration (apical margin up to 1 mm of the estimated cement-enamel junction) and MCAF along with CTG or XDM. After 6 months, the groups were compared with regard to clinical, patient-centered, and esthetic outcomes. Results: CD coverage was 72.9% for CTG versus 50.7% for XDM (P < 0.001). Recession reduction was 2.3 mm for CTG versus 1.5 mm for XDM (P < 0.001). CTG resulted in a greater increase in keratinized tissue width (CTG: 0.96 mm vs. XDM: 0.3 mm, P = 0.04) and gingival thickness (CTG: 0.9 mm vs. XDM: 0.3 mm, P < 0.001). Both treatments successfully reduced dentin hypersensitivity and increased esthetics satisfaction, with no statistically significant intergroup differences. Moreover, XDM patients experienced a shorter surgery duration (CTG: 57.2 min vs. XDM: 37.4 min, P < 0.001) and less time to no pain (visual analog scale = 0; CTG: 6.5 days vs. XDM: 3.5 days, P = 0.04). Conclusion: CTG resulted in significantly greater root coverage and increased keratinized tissue width compared to XDM for treating multiple partially restored CDs. However, increased root coverage at the CTG sites was accounted for by increased probing depth compared to the XDM sites.
Fracture resistance of weakened roots restored with relined or milled CAD-CAM glass fiber posts
(2023-01-01) Maia, Belizane Das Graças Oliveira ; Santos, Thais da Silva Alves ; Carvalho, Cláudio Antonio Talge ; Verner, Francielle Silvestre ; Junqueira, Rafael Binato ; Federal University of Juiz de Fora ; Universidade Estadual Paulista (UNESP)
This in vitro study compared the fracture resistance of weakened roots restored with milled or relined glass fiber post (GFP). Seventy bovine incisors were divided into seven groups, according to the weakening protocol (none: control; medium-weakened: MW; highly-weakened: HW) and post anatomization technique (non-relined, relined with Bulk Fill or Z350 resin; milled GFP: M). Roots were thermomechanical cycled and submitted to compression. Frequencies and groups were compared by the chi-square test and two-factor ANOVA, Tukey’s, and t-test (α=5%). The HW-M obtained the lowest values, showing significant differences compared to all groups (p<0.001). The highest value was HW-Bulk fill, with significant difference compared to MW-M (p=0.037) and HW-M (p<0.001). The frequency of repairable fractures was 100% in the control, 87%, and 63% in the MW and HW, respectively. Relined or milled GFPs can be considered to rehabilitate weakened teeth since failure patterns were predominantly repairable in all groups.

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