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MALDI–TOF bacterial subtyping for rapid detection of biomarkers in Staphylococcus aureus from subclinical bovine mastitis

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dc.contributor.authorLopes, Thainara
dc.contributor.authorFidelis, Carlos E.
dc.contributor.authorSilva, Amanda T.F.
dc.contributor.authorMota, Rinaldo A.
dc.contributor.authorRall, Vera L.M. [UNESP]
dc.contributor.authordos Santos, Marcos V.
dc.contributor.authorGonçalves, Juliano L.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionFederal Rural University of Pernambuco
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionMichigan State University
dc.date.accessioned2025-04-29T20:17:37Z
dc.date.issued2023-11-01
dc.description.abstractAims: This study aimed to evaluate matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) bacterial subtyping for the rapid detection of biomarkers in Staphylococcus aureus from subclinical bovine mastitis. Methods and results: A total of 229 S. aureus isolates were obtained from milk samples collected from cows with subclinical mastitis using microbiological culture. Staphylococcus aureus isolates were also submitted to PCR analysis targeting the mecA and mecC genes, which are indicative of methicillin resistance. Confirmation of the species was achieved through MALDI–TOF MS analysis. To analyze antimicrobial resistance patterns, the MALDI BioTyper Compass Explorer and ClinProTools Bruker software were employed, and dendrograms were generated using Bionumerics software. Conclusions: MALDI–TOF MS successfully identified S. aureus at the species level, but no methicillin resistance was observed. Moreover, spectral typing displayed limited similarity when compared to pulsed-field gel electrophoresis (PFGE).en
dc.description.affiliationSchool of Animal Science and Food Engineering University of São Paulo
dc.description.affiliationQualileite Department of Animal Nutrition and Production School of Veterinary Medicine and Animal Sciences University of São Paulo
dc.description.affiliationDepartment of Veterinary Medicine Federal Rural University of Pernambuco
dc.description.affiliationDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University
dc.description.affiliationDepartment of Large Animal Clinical Sciences College of Veterinary Medicine Michigan State University
dc.description.affiliationUnespDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2013/23613-8
dc.description.sponsorshipIdFAPESP: 2014/17411-6
dc.description.sponsorshipIdFAPESP: 2015/04570-1
dc.identifierhttp://dx.doi.org/10.1093/jambio/lxad249
dc.identifier.citationJournal of Applied Microbiology, v. 134, n. 11, 2023.
dc.identifier.doi10.1093/jambio/lxad249
dc.identifier.issn1365-2672
dc.identifier.issn1364-5072
dc.identifier.scopus2-s2.0-85178501190
dc.identifier.urihttps://hdl.handle.net/11449/310007
dc.language.isoeng
dc.relation.ispartofJournal of Applied Microbiology
dc.sourceScopus
dc.subjectbiomarkers
dc.subjectMALDI–TOF MS
dc.subjectmastitis
dc.subjectPCR
dc.subjectresistance
dc.subjectStaphylococcus aureus
dc.titleMALDI–TOF bacterial subtyping for rapid detection of biomarkers in Staphylococcus aureus from subclinical bovine mastitisen
dc.typeArtigopt
dspace.entity.typePublication
unesp.author.orcid0000-0003-2138-4982 0000-0003-2138-4982[7]

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