Effects of inclusion of the blend of essential oils, organic acids, curcumin, tannins, vitamin E, and zinc in the maternal diet, and of incubation temperature on early and late development of quail

Abstract

The maternal diet and egg incubation temperature are some of the factors that can influence the embryonic development and performance of the newly chicks at 15 d of age. This study evaluated the effects of adding a blend of organic acids, essential oils, curcumin, tannins, vitamin E, and zinc microencapsulated in to the diet of female quails (Coturnix coturnix japonica) on their productive, reproductive performance and redox parameters of their eggs and the interaction of maternal diet × incubation temperature on embryo (E16 and E18) and chicks development. At 98 d of age, 64 female quails with a mean body weight of 150 g ± 0.5 were distributed into two treatments: a BASAL diet or a diet supplemented with blend (SANNIMIX). The eggs from each female were incubated at 37.5°C (CONTROL) and 38.5°C (HIGH TEMPERATURE) throughout the incubation period. After hatching, chicks were distributed in a 2 (maternal diet) × 2 (incubation temperature) factorial design. Female quails supplemented with SANNIMIX showed better productive and reproductive performance and produced higher-quality embryos. Their offspring had greater weight at hatch and at 15 d of age. The eggs and offspring of supplemented with SANNIMIX female quails showed better oxidative stability. At E16 and E18, HIGH TEMPERATURE increased yolk sac utilization and gene expression of the growth hormone receptor (GHR). At E16, embryos from supplemented with SANNIMIX female quail had higher expression of insulin-like growth factor type I (IGFI) and heat shock protein 70 kDa genes. At 15 d of age, highest expression of the GHR and IGFI genes was observed in chicks from female quails fed the SANNIMIX diet, regardless of incubation temperature. Regarding the maternal diet × incubation temperature an improved result was observed for chicks from female quails fed with SANNIMIX even when eggs are exposed to HIGH TEMPERATURE during the incubation. The supplementation of quail diets with blend SANNIMIX improves productive and reproductive performance, egg quality and their embryos, as well as their offspring quality.