Antiarthritic and Antinociceptive Potential of Ethanolic Extract from Leaves of Doliocarpus dentatus (Aubl.) Standl. in Mouse Model

Objectives: The folk use of Doliocarpus dentatus for pain and inflammatory conditions led our group to evaluate the anti-inflammatory, antinociceptive, and antiarthritic effects of its ethanol extract from the leaves (EEDd) on mouse models. Results: Oral treatments with EEDd (100-300 mg/kg) significantly inhibited the formalin-induced nociceptive and cold sensitivity, prevented acetic acid-induced nociceptive behavior, and prevented articular inflammation (including knee edema, leukocyte infiltration, and mechanical hyperalgesia) induced by zymosan. In the peritonitis model, betulinic acid (BA, 0.3-30 mg/kg) and EEDd (300 mg/kg) significantly inhibited zymosan-induced leukocyte infiltration. In the complete Freund adjuvant (CFA) model, oral treatments with EEDd (100-300 mg/kg) for 21 days significantly inhibited mechanical hyperalgesia, cold response, and edema. In the MTT viability assay, EEDd (3-90 mu g/mL) did not induce leukocytes cytotoxicity. cytotoxicity. Most models employed male and female Swiss mice or, for the CFA test, C57BL/6 mice. Conclusion: This study demonstrates that EEDd exhibited antinociceptive, antihyperalgesic, and antiarthritic potential in mice and BA contribute for the EEDd observed activities.