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Influência do ciclo estral sobre a configuração da cromatina em vesícula germinativa (VG) de oócitos caninos

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dc.contributor.authorPereira, L. M.C. [UNESP]
dc.contributor.authorBersano, P. R.O. [UNESP]
dc.contributor.authorLopes, M. D. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-29T07:13:26Z
dc.date.available2022-04-29T07:13:26Z
dc.date.issued2013-12-01
dc.description.abstractIn bitches, oocyte maturation rates are low and the percentage of oocytes that remain in germinal vesicle (GV) independent of culture conditions is high. The objective of this study is to evaluate the configuration and positioning of chromatin in GV oocytes from bitches in anestrus and diestrus phases. The ovaries were sliced and selected only the cumulus-oocyte complex degree (COCs) I. These were subjected to hyaluronidase solution of 2% and stained with Hoechst. Were identified four stages of chromatin configuration classified as GV-1, GV-2 and GV-3 and GV-4. The analyzed data showed a significant difference (p≤0,05) between the phases demonstrating greater proportion of GV-1 and GV-2 in anoestrus and GV-3 and GV-4 in the diestrus phase.en
dc.description.affiliationUNESP, Botucatu
dc.description.affiliationDepartamento de Clínica Veterinária UNESP, Botucatu
dc.description.affiliationDepartamento de Reprodução Animal e Radiologia Veterinária UNESP, Botucatu
dc.description.affiliationUnespUNESP, Botucatu
dc.description.affiliationUnespDepartamento de Clínica Veterinária UNESP, Botucatu
dc.description.affiliationUnespDepartamento de Reprodução Animal e Radiologia Veterinária UNESP, Botucatu
dc.format.extent360-362
dc.identifier.citationActa Veterinaria Brasilica, v. 7, n. SUPPL. 1, p. 360-362, 2013.
dc.identifier.issn1981-5484
dc.identifier.scopus2-s2.0-84892686175
dc.identifier.urihttp://hdl.handle.net/11449/227479
dc.language.isopor
dc.relation.ispartofActa Veterinaria Brasilica
dc.sourceScopus
dc.subjectBitches
dc.subjectConfiguration of chromatin
dc.subjectGerminal vesicle
dc.subjectOocyte
dc.titleInfluência do ciclo estral sobre a configuração da cromatina em vesícula germinativa (VG) de oócitos caninospt
dc.title.alternativeInfluence of estrous cycle in chromatin configuration in germinal vesicle (GV) of canine oocytesen
dc.typeArtigo
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.departmentClínica Veterinária - FMVZpt
unesp.departmentReprodução Animal e Radiologia Veterinária - FMVZpt

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Botucatu - FMVZ - Faculdade de Medicina Veterinária e Zootecnia