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A stability transition at mildly acidic pH in the alpha-hemolysin (alpha-toxin) from Staphylococcus aureus

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dc.contributor.authorBortoleto, R. K.
dc.contributor.authorWard, R. J.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:02:21Z
dc.date.available2014-05-20T14:02:21Z
dc.date.issued1999-10-15
dc.description.abstractThe effects of mildly acidic conditions on the free energy of unfolding (Delta G(u)(buff)) of the pore-forming alpha-hemolysin (alpha HL) from Staphylococcus aureus were assessed between pH 5.0 and 7.5 by measuring intrinsic tryptophan fluorescence, circular dichroism and elution time in size exclusion chromatography during urea denaturation, Decreasing the pH from 7.0 to 5.0 reduced the calculated Delta G(u)(buff) from 8.9 to 4.2 kcal moI(-1), which correlates with an increased rate of pore formation previously observed over the same pH range, It is proposed that the lowered surface pH of biological membranes reduces the stability of alpha HL thereby modulating the rate of pore formation. (C) 1999 Federation of European Biochemical Societies.en
dc.description.affiliationUSP, FFCLRP, Dept Chem, Ribeirao Preto, SP, Brazil
dc.description.affiliationUNESP, IBILCE, Dept Phys, Sao Jose Rio Preto, SP, Brazil
dc.description.affiliationUnespUNESP, IBILCE, Dept Phys, Sao Jose Rio Preto, SP, Brazil
dc.format.extent438-442
dc.identifierhttp://dx.doi.org/10.1016/S0014-5793(99)01246-6
dc.identifier.citationFebs Letters. Amsterdam: Elsevier B.V., v. 459, n. 3, p. 438-442, 1999.
dc.identifier.doi10.1016/S0014-5793(99)01246-6
dc.identifier.fileWOS000083204500030.pdf
dc.identifier.issn0014-5793
dc.identifier.urihttp://hdl.handle.net/11449/21976
dc.identifier.wosWOS:000083204500030
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofFEBS Letters
dc.relation.ispartofsjr1,991
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectalpha-hemolysinpt
dc.subjectstability transitionpt
dc.titleA stability transition at mildly acidic pH in the alpha-hemolysin (alpha-toxin) from Staphylococcus aureusen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Letras e Ciências Exatas, São José do Rio Pretopt
unesp.departmentFísica - IBILCEpt

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