Publicação: Transdentinal cytotoxicity of carbodiimide (EDC) and glutaraldehyde on odontoblast-like cells
dc.contributor.author | Scheffel, Débora Lopes Salles [UNESP] | |
dc.contributor.author | Bianchi, Luciana [UNESP] | |
dc.contributor.author | Soares, Diana Gabriela [UNESP] | |
dc.contributor.author | Basso, Fernanda Gonçalves [UNESP] | |
dc.contributor.author | Sabatini, Camila [UNESP] | |
dc.contributor.author | Souza Costa, Carlos Alberto de [UNESP] | |
dc.contributor.author | Pashley, David H. | |
dc.contributor.author | Hebling, Josimeri [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Georgia Regents University | |
dc.contributor.institution | University at Buffalo | |
dc.date.accessioned | 2015-08-06T16:12:31Z | |
dc.date.available | 2015-08-06T16:12:31Z | |
dc.date.issued | 2015 | |
dc.description.abstract | Objective: To evaluate the transdentinal cytotoxicity of three different concentrations of carbodiimide (EDC) or 5% glutaraldehyde (GA) on MDPC-23 cells. Methods: Seventy 0.4-mm-thick dentin disks obtained from human molars were adapted to artificial pulp chambers. MDPC-23 cells were seeded on the pulpal surface of the disks. After 48 hours, the occlusal dentin was acid-etched and treated for 60 seconds with one of the following solutions (n=10): no treatment (negative control); 0.1 M, 0.3 M, or 0.5 M EDC; 5% GA; Sorensen buffer; or 29% hydrogen peroxide (positive control). Cell viability and morphology were assessed by methyltetrazolium assay and scanning electron microscopy (SEM), respectively. The eluates were collected after the treatments and applied on MDPC-23 seeded in a 24-well plate to analyze cell death, total protein (TP), and collagen production. The last two tests were performed 24 hours and seven days after the challenge. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests (p<0.05). Results: EDC at all test concentrations did not reduce cell viability, while 5% GA did increase cell metabolism. Cell death by necrosis was not elicited by EDC or 5% GA. At the 24-hour period, 0.3 M and 0.5 M EDC reduced TP production by 18% and 36.8%, respectively. At seven days, increased TP production was observed in all groups. Collagen production at the 24-hour period was reduced when 0.5 M EDC was used. After seven days, no difference was observed among the groups. SEM showed no alteration in cell morphology or number, except in the hydrogen peroxide group. Conclusions: Treatment of acid-etched dentin with EDC or GA did not cause transdentinal cytotoxic effects on odontoblast-like cells. | en |
dc.description.affiliation | Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Clínica Infantil, Faculdade de Odontologia de Araraquara, Araraquara, Rua Humaitá, 1680, Centro, CEP 14801-903, SP, Brasil | |
dc.description.affiliationUnesp | Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Clínica Infantil, Faculdade de Odontologia de Araraquara, Araraquara, Rua Humaitá, 1680, Centro, CEP 14801-903, SP, Brasil | |
dc.description.affiliationUnesp | Department of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, UNESP–Univ Estadual Paulista, Araraquara, São Paulo, Brazil | |
dc.description.affiliationUnesp | Department of Dental Materials and Prosthodontics, Araraquara School of Dentistry, UNESP–Univ Estadual Paulista, Araraquara, Saão Paulo, Brazil | |
dc.description.affiliationUnesp | Department of Physiology and Pathology, Araraquara School of Dentistry, UNESP–Univ Estadual Paulista, Araraquara, São Paulo, Brazil | |
dc.format.extent | 44-54 | |
dc.identifier | http://www.jopdentonline.org/doi/abs/10.2341/13-338-L | |
dc.identifier.citation | Operative Dentistry, v. 40, n. 1, p. 44-54, 2015. | |
dc.identifier.doi | 10.2341/13-338-L | |
dc.identifier.issn | 0361-7734 | |
dc.identifier.lattes | 8207097271172991 | |
dc.identifier.lattes | 4517484241515548 | |
dc.identifier.uri | http://hdl.handle.net/11449/125582 | |
dc.language.iso | eng | |
dc.relation.ispartof | Operative Dentistry | |
dc.relation.ispartofjcr | 2.130 | |
dc.relation.ispartofsjr | 1,180 | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Currículo Lattes | |
dc.subject | Glutaraldehyde | en |
dc.subject | Carbodiimide | en |
dc.subject | Cytotoxicity | en |
dc.subject | Odontoblast-like cell | en |
dc.subject | Dentin | en |
dc.title | Transdentinal cytotoxicity of carbodiimide (EDC) and glutaraldehyde on odontoblast-like cells | en |
dc.type | Artigo | |
dspace.entity.type | Publication | |
unesp.author.lattes | 8207097271172991 | |
unesp.author.lattes | 4517484241515548[6] | |
unesp.author.orcid | 0000-0002-7455-6867[6] | |
unesp.campus | Universidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquara | pt |
unesp.department | Clínica Infantil - FOAR | pt |
unesp.department | Orthodontics and Pediatric Dentistry | pt |
unesp.department | Physiology and Pathology | pt |
unesp.department | Fisiologia e Patologia - FOAR | pt |
unesp.department | Materiais Odontológicos e Prótese - FOAR | pt |