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Publicação:
Molecular detection of Paracoccidioides brasiliensis in soil

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dc.contributor.authorTheodoro, R. C.
dc.contributor.authorCandeias, JMG
dc.contributor.authorAraujo, J. P.
dc.contributor.authorBosco, SDG
dc.contributor.authorMacoris, SAD
dc.contributor.authorPadula, L. O.
dc.contributor.authorFranco, M.
dc.contributor.authorBagagli, E.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:50:40Z
dc.date.available2014-05-20T13:50:40Z
dc.date.issued2005-12-01
dc.description.abstractThe objective of the present study was to evaluate different techniques for the detection of Paracoccidioides brasiliensis in soil, e.g., culture, animal inoculation and specific DNA amplification by Nested PCR. We designed species-specific inner primers derived from rDNA regions (ITS, 5.8S gene) and found their sensitivity to be higher than culture and animal inoculation. In addition, the sensitivity of these primers was higher than p27-gene primers developed for detection of P brasiliensis in soil in a previous study. DNA from P brasiliensis was detected in soil artificially seeded with the fungus (positive soil control) and from environmental samples collected in an armadillo burrow.en
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, Inst Biociencias, Dept Microbiol & Immunol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Inst Biociencias, Dept Microbiol & Immunol, BR-18618000 Botucatu, SP, Brazil
dc.format.extent725-729
dc.identifierhttp://dx.doi.org/10.1080/13693780500129418
dc.identifier.citationMedical Mycology. Abingdon: Taylor & Francis Ltd, v. 43, n. 8, p. 725-729, 2005.
dc.identifier.doi10.1080/13693780500129418
dc.identifier.issn1369-3786
dc.identifier.lattes3320327570429539
dc.identifier.orcid0000-0003-1515-702X
dc.identifier.urihttp://hdl.handle.net/11449/18103
dc.identifier.wosWOS:000234427700008
dc.language.isoeng
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofMedical Mycology
dc.relation.ispartofjcr2.799
dc.relation.ispartofsjr0,973
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectdetectionpt
dc.subjectParacoccidioides brasiliensispt
dc.subjectPCRpt
dc.subjectsoilpt
dc.titleMolecular detection of Paracoccidioides brasiliensis in soilen
dc.typeArtigo
dcterms.licensehttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dcterms.rightsHolderTaylor & Francis Ltd
dspace.entity.typePublication
unesp.author.lattes3320327570429539
unesp.author.orcid0000-0003-1515-702X[2]
unesp.author.orcid0000-0002-9153-1485[3]
unesp.author.orcid0000-0002-6363-3740[4]
unesp.author.orcid0000-0001-5016-1046[1]
unesp.author.orcid0000-0002-8003-4109[8]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatupt
unesp.departmentMicrobiologia e Imunologia - IBBpt

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Botucatu - IBB - Instituto de Biociências