Publicação:
Fator de crescimento derivado de plaquetas humanas obtido por uma metodologia rápida e de baixo custo

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dc.contributor.authorBarbieri, C. M O [UNESP]
dc.contributor.authorCosta, Paulo Inácio da [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:21:14Z
dc.date.available2014-05-27T11:21:14Z
dc.date.issued2004-12-01
dc.description.abstractHuman platelet-derived growth factor (PDGF) was purified from lysates of clinically outdated human platelets by ionic exchange chromatography in CM-Sepharose. The eluated fraction was submitted to the Immunoblot/Slot Blot assay using anti-PDGF-AA and anti-PDGF-BB polyclonal antibodies and was evaluated as to its biological activity through the test of [H 3]-thymidine incorporation in NIH/3T3 cell line fibroblasts in culture. The Immunoblot/Slot Blot assay using anti-PDGF-AA and anti-PDGF-BB antibodies proved the presence of the PDGF in chromatographic cationic fraction. The comparison of biological activities between fiblobrast stimulation assay using recombinant PDGF-AB and partially purified PDGF was demonstrated in 165.796 and 157.567 cpm, respectively. This result, proved the potent mitogenic effect of partially purified PDGF and consequently their evidence about the wound healing activity.en
dc.description.affiliationHemonúcleo Regional de Araraquara Faculdade de Ciências Farmacêuticas UNESP, 14801-902 - Araraquara - SP
dc.description.affiliationDepartamento de Análises Clínicas Faculdade de Ciências Farmacêuticas UNESP, 14801-902 - Araraquara - SP
dc.description.affiliationUnespHemonúcleo Regional de Araraquara Faculdade de Ciências Farmacêuticas UNESP, 14801-902 - Araraquara - SP
dc.description.affiliationUnespDepartamento de Análises Clínicas Faculdade de Ciências Farmacêuticas UNESP, 14801-902 - Araraquara - SP
dc.format.extent129-134
dc.identifier.citationRevista de Ciencias Farmaceuticas, v. 25, n. 2, p. 129-134, 2004.
dc.identifier.issn0101-3793
dc.identifier.lattes6720223715917381
dc.identifier.lattes0000-0002-3350-8308
dc.identifier.scopus2-s2.0-27444441116
dc.identifier.urihttp://hdl.handle.net/11449/68055
dc.language.isopor
dc.relation.ispartofRevista de Ciencias Farmaceuticas
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectPDGF
dc.subjectPlatelets
dc.subjectWound healing
dc.subjectcation
dc.subjectplatelet derived growth factor
dc.subjectplatelet derived growth factor AA
dc.subjectplatelet derived growth factor AB
dc.subjectplatelet derived growth factor BB
dc.subjectpolyclonal antibody
dc.subjectrecombinant platelet derived growth factor
dc.subjectsepharose
dc.subjectthymidine
dc.subjecttritium
dc.subjectwound healing promoting agent
dc.subjectanimal cell
dc.subjectcell lysate
dc.subjectcell stimulation
dc.subjectcell strain 3T3
dc.subjectcontrolled study
dc.subjectdrug activity
dc.subjectdrug cost
dc.subjectdrug potency
dc.subjectdrug purification
dc.subjectdrug screening
dc.subjectfibroblast culture
dc.subjectimmunoblotting
dc.subjection exchange chromatography
dc.subjectmethodology
dc.subjectmitogenesis
dc.subjectmouse
dc.subjectnonhuman
dc.subjectslot blot hybridization
dc.subjectthrombocyte
dc.subjectwound healing
dc.titleFator de crescimento derivado de plaquetas humanas obtido por uma metodologia rápida e de baixo custopt
dc.title.alternativeHuman platelet-derived growth factor obtained by means of a fast and inexpensive methodologyen
dc.typeArtigo
dspace.entity.typePublication
unesp.author.lattes6720223715917381[2]
unesp.author.orcid0000-0002-3350-8308[2]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentAnálises Clínicas - FCFpt

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Araraquara - FCF - Faculdade de Ciências Farmacêuticas