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Taenia saginata: polymerase chain reaction for taeniasis diagnosis in human fecal samples

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dc.contributor.authorNunes, CRM
dc.contributor.authorLima, LGF
dc.contributor.authorManoel, C. S.
dc.contributor.authorPereira, R. N.
dc.contributor.authorNakano, M. M.
dc.contributor.authorGarcia, José Fernando [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-02-26T17:16:45Z
dc.date.accessioned2014-05-20T13:42:19Z
dc.date.available2014-02-26T17:16:45Z
dc.date.available2014-05-20T13:42:19Z
dc.date.issued2003-05-01
dc.description.abstractThe taeniasis-cysticercosis complex is a zoonosis of great medical and economic importance where humans play an important role as the carrier of adult stage of Taenia solium and Taenia saginata. This paper describes PCR standardization that can be applied in human fecal samples for taeniasis diagnosis. DNA extraction was achieved with DNAzol reagent, after egg disruption with glass beads. DNA prepared from fecal specimens was first purified and PCR amplified generating fragments of 170 and 600 bp. The assay described herein provides an important tool for T saginata identification in human fecal samples. (C) 2003 Elsevier B.V. (USA). All rights reserved.en
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, UNESP, Curso Med Vet, Dept Anim Hlth & Prod, BR-16050680 Aracatuba, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, UNESP, Curso Med Vet, Dept Anim Hlth & Prod, BR-16050680 Aracatuba, SP, Brazil
dc.format.extent67-69
dc.identifierhttp://dx.doi.org/10.1016/S0014-4894(03)00111-5
dc.identifier.citationExperimental Parasitology. San Diego: Academic Press Inc. Elsevier B.V., v. 104, n. 1-2, p. 67-69, 2003.
dc.identifier.doi10.1016/S0014-4894(03)00111-5
dc.identifier.issn0014-4894
dc.identifier.lattes9991374083045897
dc.identifier.urihttp://hdl.handle.net/11449/14718
dc.identifier.wosWOS:000185132300010
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofExperimental Parasitology
dc.relation.ispartofjcr1.821
dc.relation.ispartofsjr0,635
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleTaenia saginata: polymerase chain reaction for taeniasis diagnosis in human fecal samplesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dspace.entity.typePublication
unesp.author.lattes9991374083045897
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatubapt
unesp.departmentApoio, Produção e Saúde Animal - FMVApt

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Araçatuba - FMVA - Faculdade de Medicina Veterinária