Publication: Molecular and ultrastructural profiles of the symbionts in Cephalotes ants
Loading...
Date
Advisor
Coadvisor
Graduate program
Undergraduate course
Journal Title
Journal ISSN
Volume Title
Publisher
Pergamon-Elsevier B.V. Ltd
Type
Article
Access right
Acesso restrito
Abstract
The molecular and ultrastructural profiles of the symbionts found in the midgut and ileum of Cephalotes atratus, Cephalotes clypeatus, and Cephalotes pusillus were determined using the V3 region of the bacterial 16S rDNA gene and transmission electron microscopy (T.E.M.). Two samples of C atratus, three of C clypeatus, and six of C. pusillus were analyzed. The coefficients of similarity ranged from 80% to 94% for the samples of symbionts from C. clypeatus and C. atratus, despite being collected in geographically distant sites. The variability within symbionts found in the samples of C. pusillus varied from 29% to 55%, in samples geographically close as well as distant. PCR-DGGE was effective for the purpose of this study and can be considered a versatile tool to analyze gut microbiota. Details of the ultrastructural aspect of these bacteria are presented. (C) 2010 Elsevier Ltd. All rights reserved.
Description
Keywords
Bacteria, DGGE, PCR, 16S rDNA, TEM
Language
English
Citation
Micron. Oxford: Pergamon-Elsevier B.V. Ltd, v. 41, n. 5, p. 484-489, 2010.
