Publicação:
Antigen otoxicity of Agaricus blazei mushroom organic and aqueous extracts in chromosomal aberration and cytokinesis block micronucleus assays in CHO-k1 and HTC cells

dc.contributor.authorBellini, M. F.
dc.contributor.authorAngeli, JPF
dc.contributor.authorMatuo, R.
dc.contributor.authorTerezan, A. P.
dc.contributor.authorRibeiro, Lúcia Regina [UNESP]
dc.contributor.authorMantovani, M. S.
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:37:04Z
dc.date.available2014-05-20T13:37:04Z
dc.date.issued2006-04-01
dc.description.abstractAgaricus blazei (Ab) has become popularly known for its medicinal properties. Scientifically, it has been tested with regard to its capacity to protect genetic material against damage. We examined different organic extracts (methanolic extract-ME, hexanic extract-HE and n-butanolic extract-BE) and an aqueous extract (AE) of Ab, for their capacity to induce DNA damage as well as for their protective effect. Genetic damage was determined by the chromosomal aberration assay (CA) in CHO-k1 cells for all extracts and the cytokinesis block micronucleus assay (CBMN) in non drug-metabolizing (CHO-k1) and drug-metabolizing (HTC) cell lines for extract BE only. The extracts did not show clastogenicity but showed anticlastogenicity. The greatest percent reduction obtained were with BE (105%) and AE (126%) treatments in CA. BE treatment did not display genotoxicity in CHO-k1, but was genotoxic in HTC. However, BE was shown to be antigenotoxic causing decreased micronucleus frequency in HTC and CHO-k1 cells. These results suggest that all the extracts contained protective substances, but in some cases they could show a genotoxic effect with regard to metabolism. Therefore, these findings warrant caution in the use of this mushroom by the population. (c) 2005 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv Estadual Londrina, Dept Biol Geral, BR-86051990 Londrina, PR, Brazil
dc.description.affiliationUniv Fed Sao Carlos, Dept Quim, BR-13565905 Sao Carlos, SP, Brazil
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, Dept Patol, BR-18600000 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Dept Patol, BR-18600000 Botucatu, SP, Brazil
dc.format.extent355-360
dc.identifierhttp://dx.doi.org/10.1016/j.tiv.2005.08.009
dc.identifier.citationToxicology In Vitro. Oxford: Pergamon-Elsevier B.V., v. 20, n. 3, p. 355-360, 2006.
dc.identifier.doi10.1016/j.tiv.2005.08.009
dc.identifier.issn0887-2333
dc.identifier.urihttp://hdl.handle.net/11449/12793
dc.identifier.wosWOS:000236987100013
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofToxicology in Vitro
dc.relation.ispartofjcr3.105
dc.relation.ispartofsjr0,931
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectantigenotoxicitypt
dc.subjectAgaricus blazeipt
dc.subjectdrug metabolismpt
dc.subjectHTC cellspt
dc.subjectCHO-k1 cellspt
dc.subjectchromosomal aberration assaypt
dc.titleAntigen otoxicity of Agaricus blazei mushroom organic and aqueous extracts in chromosomal aberration and cytokinesis block micronucleus assays in CHO-k1 and HTC cellsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dspace.entity.typePublication
unesp.author.orcid0000-0001-7706-1379[2]
unesp.author.orcid0000-0001-5268-6508[6]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina, Botucatupt
unesp.departmentPatologia - FMBpt

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