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Proteomic-components provide insights into the defensive secretion in termite workers of the soldierless genus Ruptitermes

dc.contributor.authorCosta-Leonardo, Ana Maria [UNESP]
dc.contributor.authorda Silva, Iago Bueno [UNESP]
dc.contributor.authorPoiani, Silvana Beani [UNESP]
dc.contributor.authordos Santos-Pinto, José Roberto Aparecido [UNESP]
dc.contributor.authorEsteves, Franciele Grego [UNESP]
dc.contributor.authorda Silva, Luiza Helena Bueno [UNESP]
dc.contributor.authorPalma, Mario Sergio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-12T01:51:19Z
dc.date.available2020-12-12T01:51:19Z
dc.date.issued2020-02-20
dc.description.abstractTermite soldiers constitute the defensive frontline of the colonies, despite workers also perform such tasks, especially within the Neotropical Apicotermitinae, in which all species are soldierless. Workers of the genus Ruptitermes display an extreme form of defense, characterized by body rupture and release of a sticky secretion. Previous observations suggested that such behavior may be advantageous against enemies, but the chemical composition of this secretion has been neglected. Here we firstly provide the proteomic profile of the defensive secretion of Ruptitermes reconditus and Ruptitermes pitan workers. Additionally, the mechanisms of action of this behavior was evaluated through different bioassays. A total of 446 proteins were identified in R. reconditus and 391 proteins in R. pitan, which were classified into: toxins, defensins and proteolytic enzymes; sticky components/ alarm communication; proteins related to detoxification processes; proteins involved in folding/conformation and post-translational modifications; housekeeping proteins; and uncharacterized/hypothetical proteins. According to the bioassays, the self-sacrifice is triggered by a physical stimulus, and the defensive secretion may cause immobility and death of the opponents. Assuming that termites are abundant in the tropics and therefore exposed to predators, suicidal behaviors seem to be advantageous, since the loss of an individual benefit the whole colony. Significance: Although recent studies have reported the biochemical composition of different weapons in soldiered species of termites, such efforts had not been applied to sordierless taxa up until now. Thus, this is the first report of the defensive mechanisms in soldierless termite species based on proteomic analysis. The diversity of compounds, which included toxin-like and mucin-like proteins, reflect the mechanisms of action of the defensive secretion released by termite workers, which may cause immobility and death of the opponents. Our findings may contribute to the knowledge regarding the development of defensive strategies in termites, especially in groups which lost the soldier caste during the evolution.en
dc.description.affiliationLaboratório de Cupins Departamento de Biologia Instituto de Biociências Univ Estadual Paulista UNESP, Campus Rio Claro, Avenida 24A, 1515, Bela Vista
dc.description.affiliationCenter for the Study of Social Insects Department of Biology Institute of Biosciences of Rio Claro Univ Estadual Paulista UNESP, Rio Claro
dc.description.affiliationUnespLaboratório de Cupins Departamento de Biologia Instituto de Biociências Univ Estadual Paulista UNESP, Campus Rio Claro, Avenida 24A, 1515, Bela Vista
dc.description.affiliationUnespCenter for the Study of Social Insects Department of Biology Institute of Biosciences of Rio Claro Univ Estadual Paulista UNESP, Rio Claro
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdCNPq: 150699/2017-4
dc.description.sponsorshipIdFAPESP: 2013/26451-9
dc.description.sponsorshipIdFAPESP: 2016/16212-5
dc.description.sponsorshipIdFAPESP: 2017/10373-0
dc.description.sponsorshipIdCAPES: 206/2018
dc.description.sponsorshipIdCNPq: 301656/2013-4
dc.description.sponsorshipIdCNPq: 305539/2014-0
dc.identifierhttp://dx.doi.org/10.1016/j.jprot.2019.103622
dc.identifier.citationJournal of Proteomics, v. 213.
dc.identifier.doi10.1016/j.jprot.2019.103622
dc.identifier.issn1876-7737
dc.identifier.issn1874-3919
dc.identifier.scopus2-s2.0-85077060547
dc.identifier.urihttp://hdl.handle.net/11449/199861
dc.language.isoeng
dc.relation.ispartofJournal of Proteomics
dc.sourceScopus
dc.subjectApicotermitinae
dc.subjectIsoptera
dc.subjectLCMS-based proteomics
dc.subjectMucin-like proteins
dc.subjectSticky defensive secretion
dc.subjectToxin-like proteins
dc.titleProteomic-components provide insights into the defensive secretion in termite workers of the soldierless genus Ruptitermesen
dc.typeArtigo
dspace.entity.typePublication
unesp.author.orcid0000-0002-8874-5538 0000-0002-8874-5538[1]
unesp.author.orcid0000-0002-4584-9839[2]
unesp.author.orcid0000-0001-5099-9711[3]
unesp.author.orcid0000-0003-3172-0097[4]
unesp.author.orcid0000-0002-2027-8654[5]
unesp.author.orcid0000-0003-0595-2775[6]
unesp.author.orcid0000-0002-7363-8211[7]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Rio Claropt
unesp.departmentBiologia - IBpt

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