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Screening of supports for the immobilization of pectinmethylesterase from acerola (Malpighia glabra L)

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dc.contributor.authorde Assis, S. A.
dc.contributor.authorFernandes, P.
dc.contributor.authorFerreira, B. S.
dc.contributor.authorCabral, JMS
dc.contributor.authorOliveira, OMMF
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInst Super Tecn
dc.contributor.institutionUniv Lusofona Humanidades & Tecnol
dc.contributor.institutionBio Trend
dc.date.accessioned2014-05-20T15:22:02Z
dc.date.available2014-05-20T15:22:02Z
dc.date.issued2004-03-01
dc.description.abstractA partially purified extract of pectinmethylesterase (PME) from acerola fruit was immobilized on various supports: glass, celite, chrysotile, agarose, concanavalin A Sepharose 4B, egg shell, polyacrylamide and gelatin. In addition, reticulation with glutaraldehyde was assessed, as well as the use of gelatin in the presence of celite, glass and silica. The highest immobilization yields were obtained when the pectinmethylesterase was immobilized in concanavalin A Sepharose 4B (81.7%) and in gelatin-water (78.0%). (C) 2004 Society of Chemical Industry.en
dc.description.affiliationUNESP, Inst Quim, Dept Bioquim & Tecnol Quim, BR-14801970 Araraquara, SP, Brazil
dc.description.affiliationInst Super Tecn, Ctr Engn Biol & Quim, P-1049001 Lisbon, Portugal
dc.description.affiliationUniv Lusofona Humanidades & Tecnol, P-1749024 Lisbon, Portugal
dc.description.affiliationBio Trend, Ramada, Portugal
dc.description.affiliationUnespUNESP, Inst Quim, Dept Bioquim & Tecnol Quim, BR-14801970 Araraquara, SP, Brazil
dc.format.extent277-280
dc.identifierhttp://dx.doi.org/10.1002/jctb.973
dc.identifier.citationJournal of Chemical Technology and Biotechnology. Chichester: John Wiley & Sons Ltd, v. 79, n. 3, p. 277-280, 2004.
dc.identifier.doi10.1002/jctb.973
dc.identifier.issn0268-2575
dc.identifier.urihttp://hdl.handle.net/11449/33106
dc.identifier.wosWOS:000189185300011
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Chemical Technology and Biotechnology
dc.relation.ispartofjcr2.587
dc.relation.ispartofsjr0,766
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectacerolapt
dc.subjectpectinmethylesterasept
dc.subjectimmobilizationpt
dc.subjectkinetics characterizationpt
dc.titleScreening of supports for the immobilization of pectinmethylesterase from acerola (Malpighia glabra L)en
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Química, Araraquarapt
unesp.departmentBioquímica e Tecnologia - IQpt

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Araraquara - IQAR - Instituto de Química