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Expression of glycosylated proteins in bacterial system and purification by affinity chromatography

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dc.contributor.authorBreyer, Carlos Alexandre [UNESP]
dc.contributor.authorde Oliveira, Marcos Antonio [UNESP]
dc.contributor.authorPessoa, Adalberto
dc.contributor.editorVirgínia Picanço-Castro, Kamilla Swiech
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2022-04-29T08:14:25Z
dc.date.available2022-04-29T08:14:25Z
dc.date.issued2018-01-01
dc.description.abstractThe bacterial expression of glycoproteins has experienced significant progress in recent years, particularly in regard to the production of conjugate vaccines against pathogens. In this case, a protein carrier conjugated with glycosides is used to produce intense stimulation of the immune system. Glycoconjugate vaccines account for 35% of the global vaccine market, and consequently, several biotechnological companies have developed products for the purification of glycosylated proteins to attain homogeneity. In this chapter we present a general process for glycoprotein production in Escherichia coli and a practice method for purification of glycosylated proteins, using affinity chromatography.en
dc.description.affiliationBiosciences Institute São Paulo State University, Coastal Campus, São Vicente
dc.description.affiliationSchool of Pharmaceutical Sciences University of São Paulo
dc.description.affiliationInstituto de Biociências Universidade Estadual Paulista
dc.description.affiliationUnespBiosciences Institute São Paulo State University, Coastal Campus, São Vicente
dc.description.affiliationUnespInstituto de Biociências Universidade Estadual Paulista
dc.format.extent183-191
dc.identifierhttp://dx.doi.org/10.1007/978-1-4939-7312-5_14
dc.identifier.citationMethods in Molecular Biology, v. 1674, p. 183-191.
dc.identifier.dimensionspub.1091830954
dc.identifier.doi10.1007/978-1-4939-7312-5_14
dc.identifier.isbn978-1-4939-7311-8
dc.identifier.isbn978-1-4939-7312-5
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029
dc.identifier.orcid0000-0002-5268-8690
dc.identifier.orcid0000-0002-3602-291X
dc.identifier.orcid0000-0002-1192-7296
dc.identifier.pmid28921437
dc.identifier.scopus2-s2.0-85029700402
dc.identifier.urihttp://hdl.handle.net/11449/228390
dc.language.isoeng
dc.publisherSpringer Nature
dc.relation.ispartofMethods in Molecular Biology
dc.rights.accessRightsAcesso abertopt
dc.rights.sourceRightsoa_all
dc.rights.sourceRightsgreen
dc.sourceScopus
dc.sourceDimensions
dc.subjectAffinity chromatography
dc.subjectBacterial expression
dc.subjectConjugate vaccines
dc.subjectGlycosylated protein
dc.titleExpression of glycosylated proteins in bacterial system and purification by affinity chromatographyen
dc.typeCapítulo de livropt
dspace.entity.typePublication
relation.isOrgUnitOfPublication8fd3a51e-cf3b-42eb-b3f5-8e1f23dd0717
relation.isOrgUnitOfPublication.latestForDiscovery8fd3a51e-cf3b-42eb-b3f5-8e1f23dd0717
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, São Vicentept
unesp.departmentCiências Biológicas - IBCLPpt

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São Vicente - IBCLP - Instituto de Biociências