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Preparation of N2, N2,7-trimethylguanosine affinity columns

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dc.contributor.authorEspuny, Ruth
dc.contributor.authorBahia, Diana
dc.contributor.authorCicarelli, Regina Maria Barretto [UNESP]
dc.contributor.authorCodony, Carles
dc.contributor.authorKhaouja, Amina
dc.contributor.authorAviñó, Anna Maria
dc.contributor.authorEritja, Ramon
dc.contributor.authorBach-Elias, Montse
dc.contributor.institutionCSIC
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionHospitalet de Llobregat
dc.contributor.institutionEMBL
dc.date.accessioned2014-05-27T11:19:41Z
dc.date.available2014-05-27T11:19:41Z
dc.date.issued1999-01-01
dc.description.abstract2,2,7-trimethylguanosine (TMG) binding proteins from human cells were purified through TMG-affinity columns. TMG synthesis was improved and the TMG obtained was shown to be similar to the TMG in the 5' cap of the UsnRNAs. The eluates obtained with TMG-affinity chromatographies were very different from those isolated with m7G-affinity columns, thus suggesting that specific TMG- binding proteins were obtained. The fraction may be enriched with factors associated with import and/or hypermethylation of UsnRNPs.en
dc.description.affiliationCtro. de Invest. y Desarrollo CSIC, Jordi Girona Salgado 18-26, 08034 Barcelona
dc.description.affiliationUNESP Faculdade de Ciencias Farmaceuticas, Araraquara, SP
dc.description.affiliationDpt. de Genetica Molecular IRO (Institut de recerca oncologica) Hospitalet de Llobregat, Autovia de Castelldefels km 2.7, 08907 Barcelona
dc.description.affiliationEMBL, Meyerhofstrasse 1, Heidelberg
dc.description.affiliationUnespUNESP Faculdade de Ciencias Farmaceuticas, Araraquara, SP
dc.format.extent125-136
dc.identifierhttp://dx.doi.org/10.1080/07328319908045599
dc.identifier.citationNucleosides and Nucleotides, v. 18, n. 1, p. 125-136, 1999.
dc.identifier.doi10.1080/07328319908045599
dc.identifier.issn0732-8311
dc.identifier.scopus2-s2.0-0032589080
dc.identifier.urihttp://hdl.handle.net/11449/65686
dc.language.isoeng
dc.relation.ispartofNucleosides and Nucleotides
dc.rights.accessRightsAcesso restritopt
dc.sourceScopus
dc.subjectbinding protein
dc.subjectguanosine derivative
dc.subjectsmall nuclear RNA
dc.subjectantibody
dc.subjectcarrier protein
dc.subjectdrug derivative
dc.subjectfungal protein
dc.subjectguanosine
dc.subjecthemocyanin
dc.subjectkeyhole limpet hemocyanin
dc.subjectkeyhole-limpet hemocyanin
dc.subjectmonoclonal antibody
dc.subjectN(2),N(2),7 trimethylguanosine
dc.subjectN(2),N(2),7-trimethylguanosine
dc.subjectnuclear protein
dc.subjectsepharose
dc.subjectserum albumin
dc.subjectaffinity chromatography
dc.subjectanimal experiment
dc.subjectextraction
dc.subjecthuman
dc.subjectmouse
dc.subjectnonhuman
dc.subjectnucleotide metabolism
dc.subjectprotein methylation
dc.subjectprotein purification
dc.subjectspliceosome
dc.subjectsynthesis
dc.subjectanimal
dc.subjectcell nucleus
dc.subjectchemistry
dc.subjectcytoplasm
dc.subjectHeLa cell
dc.subjectinstrumentation
dc.subjectisolation and purification
dc.subjectmethodology
dc.subjectrabbit
dc.subjectSaccharomyces cerevisiae
dc.subjectAnimals
dc.subjectAntibodies
dc.subjectAntibodies, Monoclonal
dc.subjectCarrier Proteins
dc.subjectCell Nucleus
dc.subjectChromatography, Affinity
dc.subjectCytoplasm
dc.subjectFungal Proteins
dc.subjectGuanosine
dc.subjectHela Cells
dc.subjectHemocyanin
dc.subjectHumans
dc.subjectMice
dc.subjectNuclear Proteins
dc.subjectRabbits
dc.subjectSepharose
dc.subjectSerum Albumin
dc.titlePreparation of N2, N2,7-trimethylguanosine affinity columnsen
dc.typeArtigopt
dcterms.licensehttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dspace.entity.typePublication
relation.isDepartmentOfPublication5004bcab-94af-4939-b980-091ae9d0a19e
relation.isDepartmentOfPublication.latestForDiscovery5004bcab-94af-4939-b980-091ae9d0a19e
relation.isOrgUnitOfPublication95697b0b-8977-4af6-88d5-c29c80b5ee92
relation.isOrgUnitOfPublication.latestForDiscovery95697b0b-8977-4af6-88d5-c29c80b5ee92
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentCiências Biológicas - FCFpt

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Araraquara - FCF - Faculdade de Ciências Farmacêuticas