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Identification of Corynebacterium bovis by MALDI-mass spectrometry

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dc.contributor.authorLangoni, Hello [UNESP]
dc.contributor.authorCamargo da Silva, Carolina Polo [UNESP]
dc.contributor.authorTroncarelli, Marcella Zampoli
dc.contributor.authorTata, Alessandra
dc.contributor.authorAnacleto Belaz, Katia Roberta
dc.contributor.authorEberlin, Marcos Nogueira
dc.contributor.authorJoaquim, Samea Fernandes [UNESP]
dc.contributor.authorGuimaraes, Felipe Freitas [UNESP]
dc.contributor.authorPardo, Renata Bonini
dc.contributor.authorGomes, Eduardo Nardini [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInst Fed Catarinense
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionFac Tecnol Marilia
dc.date.accessioned2018-11-26T17:32:57Z
dc.date.available2018-11-26T17:32:57Z
dc.date.issued2017-06-01
dc.description.abstractCorynebacterium bovis is a mastitis-causing microorganism responsible for economic losses related to decrease in milk production. The aim of the study was identify Corynebacterium, spp. strains recovered from milk samples of subclinical mastitis by using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Samples were collected during a 10-mo mastitis-monitoring program in a high-production dairy farm. In this study, 80 strains were analyzed; from these 54 (67.5%) were identified at species level as Corynebacterium bovis, 24 (31.2%) isolates were identified at the genus level as Corynebacterium spp., and only 1 (1.35%) isolated had unreliable identification. Results demonstrated that MALDI-MS could be an important technique for the identification of Corynebacterium spp. in milk.en
dc.description.affiliationUniv Estadual Paulista, Fac Med Vet & Zootecnia, Dept Higiene Vet & Saude Publ, Campus Botucatu,Distrito de Rubiao Junior S-N, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationInst Fed Catarinense, Rodovia SC-283 Km 08 Fragosos, Concordia, SC 89703720, Brazil
dc.description.affiliationUniv Estadual Campinas, Inst Chem, Thomson Mass Spectrometry Lab, BR-13083859 Campinas, SP, Brazil
dc.description.affiliationFac Tecnol Marilia, Ave Castro Alves,62 Somenzari, BR-17500000 Marilia, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Engn Agron & Engn Pesca, Campus Registro,Ave Nelson Brihi Badur, BR-11900000 Registro, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med Vet & Zootecnia, Dept Higiene Vet & Saude Publ, Campus Botucatu,Distrito de Rubiao Junior S-N, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Engn Agron & Engn Pesca, Campus Registro,Ave Nelson Brihi Badur, BR-11900000 Registro, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2012/24213-0
dc.format.extent4287-4289
dc.identifierhttp://dx.doi.org/10.3168/jds.2016-11922
dc.identifier.citationJournal Of Dairy Science. New York: Elsevier Science Inc, v. 100, n. 6, p. 4287-4289, 2017.
dc.identifier.doi10.3168/jds.2016-11922
dc.identifier.issn0022-0302
dc.identifier.lattes3595538854439331
dc.identifier.orcid0000-0002-2712-5685
dc.identifier.urihttp://hdl.handle.net/11449/162815
dc.identifier.wosWOS:000401783800011
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal Of Dairy Science
dc.relation.ispartofsjr1,350
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectmastitis
dc.subjectCorynebacterium bovis
dc.subjectproteomic characterization
dc.titleIdentification of Corynebacterium bovis by MALDI-mass spectrometryen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dspace.entity.typePublication
unesp.author.lattes3595538854439331[10]
unesp.author.orcid0000-0002-2712-5685[10]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.departmentEngenharia Agronômica - FCAVRpt
unesp.departmentHigiene Veterinária e Saúde Pública - FMVZpt

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Coleções

Botucatu - FMVZ - Faculdade de Medicina Veterinária e Zootecnia
Registro - FCAVR - Faculdade de Ciências Agrárias do Vale do Ribeira