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Publicação:
Effect of surface characteristics of soft liners and tissue conditioners and saliva on the adhesion and biofilm formation

dc.contributor.authorDe Foggi, Camila Cristina [UNESP]
dc.contributor.authorAyres, Marina Siqueira Bambini [UNESP]
dc.contributor.authorFeltrin, Guilherme Paladini [UNESP]
dc.contributor.authorJorge, Janaina Habib [UNESP]
dc.contributor.authorMachado, Ana Lúcia [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:19:20Z
dc.date.available2018-12-11T17:19:20Z
dc.date.issued2018-02-01
dc.description.abstractPurpose: To investigate the influence of surface characteristics and saliva on the adhesion and biofilm formation of Candida glabrata and methicillin-resistant Staphylococcus aureus (MRSA) to soft liners and tissue conditioners. Methods: For each material (Ufi Gel P - UG; Sofreliner S - SS; Trusoft - TR; Coe Comfort - CC; Softone - ST), specimens were prepared and roughness (Ra), hydrophobicity (water contact angles-WCA) and surface free energy (SFE) were measured. Surface morphology was also analyzed using scanning electron microscopy (SEM). Specimens were incubated in C. glabrata or MRSA suspensions for 90 minutes (adhesion) or 48 hours (biofilm). The absorbance (AB) was measured by XTT assay. Experiments were performed using specimens that were either uncoated or had been coated with saliva. Data were analyzed using one- or two-way ANOVAs, followed by Tukey's test (a= 0.05). Results: TR exhibited the highest Ra and UG the lowest. SEM images also showed that UG and SS had smooth surfaces, while TR presented several irregularities and pores. In the absence of saliva, UG and SS presented higher WCA and lower SFE than the other materials. XTT results showed that, in the C. glabrata adhesion assay, the AB value was higher for TR followed by UG > CC > SS > ST. For the biofilm formation of C. glabrata, AB values were in the following order TR > CC = UG > ST = SS. In the adhesion assay, AB values obtained for MRSA were TR > UG = CC > ST > SS and for the biofilm formation were TR > ST > CC > UG > SS. Saliva decreased the WCA and increased the SFE for all materials. In general, the presence of saliva decreased the adhesion and biofilm formation of both microorganisms to the acrylic-based material (TR) and tissue conditioners (CC and ST), and increased for the siliconebased soft liners (UH and SS). Surface characteristics and the influence of saliva varied among materials. Roughness seemed to favor C. glabrata and MRSA adhesion and biofilm formation.en
dc.description.affiliationDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) Dental School Campus Araraquara, R. Humaitá, no. 1680
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) Dental School Campus Araraquara, R. Humaitá, no. 1680
dc.format.extent45-52
dc.identifier.citationAmerican Journal of Dentistry, v. 31, n. 1, p. 45-52, 2018.
dc.identifier.issn0894-8275
dc.identifier.scopus2-s2.0-85045184482
dc.identifier.urihttp://hdl.handle.net/11449/176163
dc.language.isoeng
dc.relation.ispartofAmerican Journal of Dentistry
dc.relation.ispartofsjr0,509
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.titleEffect of surface characteristics of soft liners and tissue conditioners and saliva on the adhesion and biofilm formationen
dc.typeArtigo
dspace.entity.typePublication
unesp.author.lattes8000248781842587[5]
unesp.author.orcid0000-0002-2718-428X[5]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquarapt
unesp.departmentMateriais Odontológicos e Prótese - FOARpt

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