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Effect of hydrogen-peroxide-mediated oxidative stress on human dental pulp cells

dc.contributor.authorSoares, Diana Gabriela de Souza [UNESP]
dc.contributor.authorBasso, Fernanda Gonçalves [UNESP]
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorCosta, Carlos Alberto de Souza[UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-08-06T16:12:30Z
dc.date.available2015-08-06T16:12:30Z
dc.date.issued2015
dc.description.abstractTo evaluate the effect of the oxidative stress on human dental pulp cells (HDPCs) promoted by toxic concentrations of hydrogen peroxide (H2O2) on its odontoblastic differentiation capability through time. Methods HDPCs were exposed to two different concentrations of H2O2 (0.1 and 0.3 μg/ml) for 30 min. Thereafter, cell viability (MTT assay) and oxidative stress generation (H2DCFDA fluorescence assay) were immediately evaluated. Data were compared with those for alkaline phosphatase (ALP) activity (thymolphthalein assay) and mineralized nodule deposition (alizarin red) by HDPCs cultured for 7 days in osteogenic medium. Results A significant reduction in cell viability and oxidative stress generation occurred in the H2O2-treated cells when compared with negative controls (no treatment), in a concentration-dependent fashion. Seven days after H2O2 treatment, the cells showed significant reduction in ALP activity compared with negative control and no mineralized nodule deposition. Conclusion Both concentrations of H2O2 were toxic to the cells, causing intense cellular oxidative stress, which interfered with the odontogenic differentiation capability of the HDPCs. Clinical significance The intense oxidative stress on HDPCs mediated by H2O2 at toxic concentrations promotes intense reduction on odontoblastic differentiation capability in a 7-day evaluation period, which may alter the initial pulp healing capability in the in vivo situation.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Clínica Infantil, Faculdade de Odontologia de Araraquara, Araraquara, Rua Humaitá, 1680, Centro, CEP 14801-903, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Clínica Infantil, Faculdade de Odontologia de Araraquara, Araraquara, Rua Humaitá, 1680, Centro, CEP 14801-903, SP, Brasil
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics, Univ. Estadual Paulista – UNESP, Araraquara School of Dentistry, Araraquara, SP, Brazil
dc.description.affiliationUnespDepartment of Physiology and Pathology, Univ. Estadual Paulista – UNESP, Araraquara School of Dentistry, Araraquara, SP, Brazil
dc.format.extent750-756
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0300571214003443
dc.identifier.citationJournal of Dentistry, v. 43, n. 6, p. 750-756, 2015.
dc.identifier.doi10.1016/j.jdent.2014.12.006
dc.identifier.issn0300-5712
dc.identifier.lattes8207097271172991
dc.identifier.urihttp://hdl.handle.net/11449/125581
dc.language.isoeng
dc.relation.ispartofJournal of Dentistry
dc.relation.ispartofjcr3.770
dc.relation.ispartofsjr1,919
dc.rights.accessRightsAcesso restritopt
dc.sourceCurrículo Lattes
dc.subjectPulp cellsen
dc.subjectHydrogen peroxideen
dc.titleEffect of hydrogen-peroxide-mediated oxidative stress on human dental pulp cellsen
dc.typeArtigopt
dspace.entity.typePublication
relation.isDepartmentOfPublication3936e2e2-946a-42ab-8b9d-9521513200fc
relation.isDepartmentOfPublicationb3ba3d9c-022e-4521-8805-0bcceea7372e
relation.isDepartmentOfPublication.latestForDiscovery3936e2e2-946a-42ab-8b9d-9521513200fc
relation.isOrgUnitOfPublicationca4c0298-cd82-48ee-a9c8-c97704bac2b0
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unesp.author.lattes8207097271172991
unesp.author.lattes4517484241515548[4]
unesp.author.orcid0000-0002-7455-6867[4]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquarapt
unesp.departmentClínica Infantil - FOARpt
unesp.departmentFisiologia e Patologia - FOARpt
unesp.departmentMateriais Odontológicos e Prótese - FOARpt

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