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Screening of genomic libraries

dc.contributor.authorNovelli, Valdenice M.
dc.contributor.authorCristofani-Yaly, Mariângela
dc.contributor.authorBastianel, Marinês
dc.contributor.authorPalmieri, Dario A. [UNESP]
dc.contributor.authorMachado, Marcos A.
dc.contributor.institutionAgência Paulista de Tecnologia dos Agronegócios (APTA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:29:29Z
dc.date.available2014-05-27T11:29:29Z
dc.date.issued2013-05-10
dc.description.abstractMicrosatellites, or simple sequence repeats (SSRs), have proven to be an important molecular marker in plant genetics and breeding research. The main strategies to obtain these markers can be through genomic DNA and from expressed sequence tags (ESTs) from mRNA/cDNA libraries. Genetic studies using microsatellite markers have increased rapidly because they can be highly polymorphic, codominant markers and they show heterozygous conserved sequences. Here, we describe a methodology to obtain microsatellite using the enrichment library of DNA genomic sequences. This method is highly efficient to development microsatellite markers especially in plants that do not have available ESTs or genome databases. This methodology has been used to enrich SSR marker libraries in Citrus spp., an important tool to genotype germplasm, to select zygotic hybrids, and to saturate genetic maps in breeding programs. © Springer Science+Business Media, LLC 2013.en
dc.description.affiliationInstituto Agronômico Centro APTA Citros Sylvio Moreira, Cordeirópolis SP
dc.description.affiliationFCL-UNESP - Assis, Assis SP
dc.description.affiliationUnespFCL-UNESP - Assis, Assis SP
dc.format.extent17-24
dc.identifierhttp://dx.doi.org/10.1007/978-1-62703-389-3_2
dc.identifier.citationMethods in Molecular Biology, v. 1006, p. 17-24.
dc.identifier.doi10.1007/978-1-62703-389-3_2
dc.identifier.issn1064-3745
dc.identifier.scopus2-s2.0-84877103470
dc.identifier.urihttp://hdl.handle.net/11449/75377
dc.language.isoeng
dc.relation.ispartofMethods in Molecular Biology
dc.relation.ispartofsjr0,616
dc.rights.accessRightsAcesso restritopt
dc.sourceScopus
dc.subjectEnrichment methods
dc.subjectMicrosatellites
dc.subjectMolecular markers
dc.subjectgenomic DNA
dc.subjectCitrus
dc.subjectcodominance
dc.subjectDNA library
dc.subjectexpressed sequence tag
dc.subjectgene library
dc.subjectgene mapping
dc.subjectgene sequence
dc.subjectgenetic screening
dc.subjectgenotype
dc.subjectgermplasm
dc.subjectheterozygote
dc.subjecthybrid
dc.subjectmicrosatellite marker
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectplant breeding
dc.subjectpriority journal
dc.subjectsimple sequence repeat
dc.subjectBreeding
dc.subjectComputational Biology
dc.subjectDNA, Plant
dc.subjectGenetic Markers
dc.subjectGenome, Plant
dc.subjectGenomic Library
dc.subjectMicrosatellite Repeats
dc.titleScreening of genomic librariesen
dc.typeArtigopt
dcterms.licensehttp://www.springer.com/open+access/authors+rights
dspace.entity.typePublication
relation.isOrgUnitOfPublicationc3f68528-5ea8-4b32-a9f4-3cfbd4bba64d
relation.isOrgUnitOfPublication.latestForDiscoveryc3f68528-5ea8-4b32-a9f4-3cfbd4bba64d
unesp.author.orcid0000-0003-0841-6257[4]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências e Letras, Assispt

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