Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate
dc.contributor.author | Queiroz Santos, Vidiany Aparecida [UNESP] | |
dc.contributor.author | Del Bianchi, Vanildo Luiz [UNESP] | |
dc.contributor.author | Garcia-Cruz, Crispin Humberto [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2014-12-03T13:11:08Z | |
dc.date.available | 2014-12-03T13:11:08Z | |
dc.date.issued | 2014-01-01 | |
dc.description.abstract | Zymomonas mobilis was immobilized using a cell suspension fixed to 8.6 x 10(7) CFU mL(-1) by spectrophotometry. This biomass was suspended in sodium alginate solution (3%) that was dropped with a hypodermic syringe into 0.2 M calcium chloride solution. Was test two initial pH of fermentation medium (4 and 5) and different sucrose concentrations 15, 20, 25, 30 and 35% at 30 degrees C, without stirring for 24, 48, 72 and 96 hours. The levan production to pH 4 was high in sucrose 25% for 24 (16.51 g L-1) and 48 (15.31 g L-1) hours. The best values obtained to pH 5 was in sucrose 35% during 48 (22.39 g L-1) and 96 (23.5 g L-1) hours, respectively. The maximum levan yield was 40.8% and 22.47% in sucrose 15% to pH 4 and 5, respectively. Substrate consumption to pH 4 was bigger in sucrose 15 (56.4%) and 20% (59.4%) and to pH 5 was in 25 (68.85%) and 35% (64.64%). In relation to immobilization efficiency, Zymomonas mobilis showed high adhesion and colonization in support, indicated by cell growth increased from 107 to 10(9) CFU mL(-1) during fermentation time. | en |
dc.description.affiliation | Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas Sao Jos, Dept Engn & Tecnol Alimentos, BR-15054000 Sao Paulo, Brazil | |
dc.description.affiliationUnesp | Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas Sao Jos, Dept Engn & Tecnol Alimentos, BR-15054000 Sao Paulo, Brazil | |
dc.format.extent | 349-354 | |
dc.identifier | http://dx.doi.org/10.4025/actascitechnol.v36i2.18372 | |
dc.identifier.citation | Acta Scientiarum-technology. Maringa: Univ Estadual Maringa, Pro-reitoria Pesquisa Pos-graduacao, v. 36, n. 2, p. 349-354, 2014. | |
dc.identifier.doi | 10.4025/actascitechnol.v36i2.18372 | |
dc.identifier.file | WOS000334270000022.pdf | |
dc.identifier.issn | 1806-2563 | |
dc.identifier.lattes | 9441894011582350 | |
dc.identifier.lattes | 3761109299906668 | |
dc.identifier.uri | http://hdl.handle.net/11449/112885 | |
dc.identifier.wos | WOS:000334270000022 | |
dc.language.iso | eng | |
dc.publisher | Univ Estadual Maringa, Pro-reitoria Pesquisa Pos-graduacao | |
dc.relation.ispartof | Acta Scientiarum: Technology | |
dc.relation.ispartofjcr | 0.231 | |
dc.relation.ispartofsjr | 0,168 | |
dc.rights.accessRights | Acesso aberto | |
dc.source | Web of Science | |
dc.subject | entrapment cell | en |
dc.subject | batch fermentation | en |
dc.subject | Biopolymer | en |
dc.title | Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate | en |
dc.type | Artigo | |
dcterms.rightsHolder | Univ Estadual Maringa, Pro-reitoria Pesquisa Pos-graduacao | |
dspace.entity.type | Publication | |
unesp.author.lattes | 9441894011582350 | |
unesp.author.lattes | 3761109299906668[3] | |
unesp.campus | Universidade Estadual Paulista (UNESP), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Preto | pt |
unesp.department | Engenharia e Tecnologia de Alimentos - IBILCE | pt |
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