Publicação: A simplified spectrophotometric method for routine analysis of saccharin in commercial noncaloric sweeteners
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A simple, rapid, and sensitive spectrophotometric method for routine analysis of saccharin in commercial noncaloric sweeteners is proposed. This method is based on the reaction of saccharin with tetrachloro-p-benzoquinone (p-chloranil) accelerated by hydrogen peroxide and conducted in an ethanol:acetone (4:1) medium, producing a violet-red compound (γ max = 550 nm). Beer's law is obeyed in a concentration range of 2.05 × 10 -4 to 3.00 × 10 -3 M with an excellent correlation coefficient (r = 0.9998). The detection limit was 1.55 × 10 -5 M, and the effect of interferences on the spectrophotometric measurements was evaluated. The proposed procedure was applied successfully to the determination of saccharin in noncaloric sweeteners. Recoveries were within 99.2-104.3% with standard deviations ranging from to 0.5-1.6%. Results of the proposed method compare very favorably with those given by the high-performance liquid chromatography method recommended by the Food and Drug Administration.
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Noncaloric sweeteners, Saccharin, Spectrophotometric determination, 4 chloroaniline, acetone, alcohol, hydrogen peroxide, saccharin, sweetening agent, absorption, chemical reaction, concentration (parameters), correlation coefficient, high performance liquid chromatography, intermethod comparison, molecular interaction, reaction analysis, reaction time, reliability, spectrophotometry, Chloranil, Hydrogen Peroxide, Indicators and Reagents, Sensitivity and Specificity, Spectrophotometry, Sweetening Agents, Viola (angiosperm)
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Inglês
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Journal of Agricultural and Food Chemistry, v. 52, n. 26, p. 7788-7792, 2004.
