Publicação: Bifunctional silica nanoparticles for the exploration of biofilms of Pseudomonas aeruginosa
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Data
2013-08-01
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Coorientador
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Luminescent silica nanoparticles are frequently employed for biotechnology applications mainly because of their easy functionalization, photo-stability, and biocompatibility. Bifunctional silica nanoparticles (BSNPs) are described here as new efficient tools for investigating complex biological systems such as biofilms. Photoluminescence is brought about by the incorporation of a silylated ruthenium(II) complex. The surface properties of the silica particles were designed by reaction with amino-organosilanes, quaternary ammonium-organosilanes, carboxylate-organosilanes and hexamethyldisilazane. BSNPs were characterized extensively by DRIFT, 13C and 29Si solid state NMR, XPS, and photoluminescence. Zeta potential and contact angle measurements exhibited various surface properties (hydrophilic/hydrophobic balance and electric charge) according to the functional groups. Confocal laser scanning microscopy (CLSM) measurements showed that the spatial distribution of these nanoparticles inside a biofilm of Pseudomonas aeruginosa PAO1 depends more on their hydrophilic/hydrophobic characteristics than on their size. CLSM observations using two nanosized particles (25 and 68 nm) suggest that narrow diffusion paths exist through the extracellular polymeric substances matrix. © 2013 Copyright Taylor and Francis Group, LLC.
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bifunctional silica nanoparticles, P. aeruginosa (PAO1) biofilms, photoluminescence, surface modification, biofilm, biotechnology, diffusion, functional group, luminescence, microbial community, microscopy, nanotechnology, particle size, polymer, reaction kinetics, ruthenium, silica, Pseudomonas aeruginosa
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Inglês
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Biofouling, v. 29, n. 7, p. 775-788, 2013.
